Structure of PDB 2dkh Chain A

Receptor sequence
>2dkhA (length=614) Species: 285 (Comamonas testosteroni) [Search protein sequence]
MQFHLNGFRPGNPLIAPASPLAPAHTEAVPSQVDVLIVGCGPAGLTLAAQ
LAAFPDIRTCIVEQKEGPMELGQADGIACRTMEMFEAFEFADSILKEACW
INDVTFWKPDPGQPGRIARHGRVQDTEDGLSEFPHVILNQARVHDHYLER
MRNSPSRLEPHYARRVLDVKVDHGAADYPVTVTLERCDAAHAGQIETVQA
RYVVGCDGARSNVRRAIGRQLVGDSANQAWGVMDVLAVTDFPDVRYKVAI
QSEQGNVLIIPREGGHLVRFYVEMDNITVEQLIATAQRVLHPYKLEVKNV
PWWSVYEIGQRICAKYDDVVDAVATPDSPLPRVFIAGDACHTHSPKAGQG
MNFSMQDSFNLGWKLAAVLRKQCAPELLHTYSSERQVVAQQLIDFDREWA
KDPKEFQKYFEQHGRFTAGVGTHYAPSLLTGQAKHQALASGFTVGMRFHS
APVVRVCDAKPVQLGHCGKADGRWRLYAFAAQNDLAQPESGLLALCRFLE
GDAASPLRRFTPAGQDIDSIFDLRAVFPQAYTEVALETLPALLLPPKGQL
GMIDYEKVFSPDLKNAGQDIFELRGIDRQQGALVVVRPDQYVAQVLPLGD
HAALSAYFESFMRA
3D structure
PDB2dkh Crystal structure of 3-hydroxybenzoate hydroxylase from Comamonas testosteroni has a large tunnel for substrate and oxygen access to the active site
ChainA
Resolution1.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D75 R262 Y271 P356
Catalytic site (residue number reindexed from 1) D75 R262 Y271 P345
Enzyme Commision number 1.14.13.23: 3-hydroxybenzoate 4-monooxygenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD A P42 A43 E63 Q64 Q73 R165 V166 D207 G208 V232 R269 Y271 Y317 G348 D349 P42 A43 E63 Q64 Q73 R165 V166 D207 G208 V232 R269 Y271 Y306 G337 D338
BS02 3HB A D75 H135 K247 I260 Y271 D75 H135 K247 I260 Y271
Gene Ontology
Molecular Function
GO:0016491 oxidoreductase activity
GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0018668 3-hydroxybenzoate 4-monooxygenase activity
GO:0042803 protein homodimerization activity
GO:0071949 FAD binding

View graph for
Molecular Function
External links
PDB RCSB:2dkh, PDBe:2dkh, PDBj:2dkh
PDBsum2dkh
PubMed17045293
UniProtQ6SSJ6|MOBA_COMTE 3-hydroxybenzoate 4-monooxygenase (Gene Name=mobA)

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