Structure of PDB 2cgl Chain A

Receptor sequence

>2cglA (length=479) Species: 469008 (Escherichia coli BL21(DE3))

TFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHSQNGYVTW
DVDSLESAIRLGLNKVCAAGIAIDSIGIDTWGVDFVLLDQQGQRVGLPVA
YRDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELI
PHIAHALLMPDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSG
ANKAWFGRPTHPGNVIGHWICPQGNEIPVVAVASHDTASAVIASPLNGSR
AAYLSSGTWSLMGFESQTPFTNDTALAANITNEGGAEGRYRVLKNIMGLW
LLQRVLQERQINDLPALIAATQALPACRFIINPNDDRFINPDEMCSEIQA
ACREMAQPIPESDAELARCIFDSLALLYADVLHELAQLRGEDFSQLHIVG
GGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFR
QVVSTTANLTTFTPNPDSEIAHYVALIHS

3D structure

• PDB: 2cgl Structure and Reaction Mechanism of L-Rhamnulose Kinase from Escherichia Coli.
• Chain: A
• Resolution: 1.88 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.5: rhamnulokinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	LFR	A	W82 G83 H236 D237 W260 N296	W81 G82 H235 D236 W259 N295
BS02	ADP	A	S14 R17 G258 T259 Q304 L315 G402 Q405 N406	S13 R16 G257 T258 Q303 L314 G401 Q404 N405
BS03	PO3	A	G12 T259	G11 T258

Gene Ontology

Molecular Function

• GO:0004370 glycerol kinase activity
• GO:0005524 ATP binding
• GO:0008993 rhamnulokinase activity
• GO:0016301 kinase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0006071 glycerol metabolic process
• GO:0016310 phosphorylation
• GO:0019299 rhamnose metabolic process
• GO:0019301 rhamnose catabolic process
• GO:0046835 carbohydrate phosphorylation

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:2cgl, PDBe:2cgl, PDBj:2cgl
• PDBsum: 2cgl
• PubMed: 16674975
• UniProt: P32171|RHAB_ECOLI L-Rhamnulokinase (Gene Name=rhaB)