Structure of PDB 2bvl Chain A

Receptor sequence

>2bvlA (length=543) Species: 1496 (Clostridioides difficile)

MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVEKYLKLK
DINSLTDICIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHF
VWIGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAI
NDTLESFRENLNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPEL
IIDDIVKTYLSNEYSKEIDELNTYIEESLNKITQNSGNDVRNFGEFKNGE
SFNLYEQELVERWNLAAASDILRISALKEIGGMYLDVDMLPGIQPDLFES
IEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEHFDMLDEEVQSSFESVL
ASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKDSYCSNLIV
KQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRN
FEISKTNISQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGAL

3D structure

• PDB: 2bvl Structural Basis for the Function of Clostridium Difficile Toxin B.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.-
  3.4.22.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	A	D288 E515	D288 E515
BS02	UDP	A	V101 W102 I103 N139 L265 S269 D286 S518 L519 W520	V101 W102 I103 N139 L265 S269 D286 S518 L519 W520
BS03	GLC	A	D270 R273 D286 I383 N384 W520	D270 R273 D286 I383 N384 W520
BS04	TBR	A	D29 E32 E33	D29 E32 E33
BS05	TBR	A	D310 N378 S379 K380 E515 M516	D310 N378 S379 K380 E515 M516
BS06	TBR	A	E77 V80 I494 A496 D497	E77 V80 I494 A496 D497
BS07	TBR	A	R173 N425 T428 N429 R455	R173 N425 T428 N429 R455

Gene Ontology

Molecular Function

• GO:0016757 glycosyltransferase activity

External links

• PDB: RCSB:2bvl, PDBe:2bvl, PDBj:2bvl
• PDBsum: 2bvl
• PubMed: 16054646
• UniProt: P18177|TCDB_CLODI Toxin B (Gene Name=tcdB)