Structure of PDB 2bn4 Chain A

Receptor sequence

>2bn4A (length=643) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]

NRDIAQVVTENNKNYLVLYASQTGTAEDYAKKFSKELVAKFNLNVMCADV
ENYDFESLNDVPVIVSIFISTYGEGDFPDGAVNFEDFICNAEAGALSNLR
YNMFGLGNSTYEFFNGAAKKAEKHLSAAGAIRLGKLGEADDGAGTTDEDY
MAWKDSILEVLKDELHLDEQEAKFTSQFQYTVLNEITDSMSLGEPSAHYL
PSHNRNADGIQLGPFDLSQPYIAPIVKSRELFSSNDRNCIHSEFDLSGSN
IKYSTGDHLAVWPSNPLEKVEQFLSIFNLDPETIFDLKPLDPTVKVPFPT
PTTIGAAIKHYLEITGPVSRQLFSSLIQFAPNADVKEKLTLLSKDKDQFA
VEITSKYFNIADALKYLSDGAKWDTVPMQFLVESVPQMTPRYYSISSSSL
SEKQTVHVTSIVENFPNPELPDAPPVVGVTTNLLRNIQLAQNNVNIAETN
LPVHYDLNGPRKLFANYKLPVHVRRSNFRLPSNPSTPVIMIGPGTGVAPF
RGFIRERVAFLESQKKGGNNVSLGKHILFYGSRNTDDFLYQDEWPEYAKK
LDGSFEMVVAHSRLPNTKKVYVQDKLKDYEDQVFEMINNGAFIYVCGDAK
GMAKGVSTALVGILSRGKSITTDEATELIKMLKTSGRYQEDVW

3D structure

PDB

2bn4 A Second Fmn-Binding Site in Yeast Nadph-Cytochrome P450 Reductase Suggests a Mechanism of Electron Transfer by Diflavin Reductases.

Chain

Resolution

2.91 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	Y441 S442 C644 D689 W691
Catalytic site (residue number reindexed from 1)	Y393 S394 C596 D641 W643
Enzyme Commision number	1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	FAD	A	G364 P365 F406 N407 R439 Y440 Y441 S442 S458 I459 E461 V474 V475 G476 V477 T478 T479 T543 W691	G316 P317 F358 N359 R391 Y392 Y393 S394 S410 I411 E413 V426 V427 G428 V429 T430 T431 T495 W643
BS02	FMN	A	S67 Q68 T69 T71 S116 T117 Y118 L152 G153 N154 Y157 F159 F160 N161 V690	S21 Q22 T23 T25 S70 T71 Y72 L106 G107 N108 Y111 F113 F114 N115 V642
BS03	NAP	A	I459 E461 T543 S580 S610 R611 K617 Y619 Q621 D646 K648 G649 M650 W691	I411 E413 T495 S532 S562 R563 K569 Y571 Q573 D598 K600 G601 M602 W643

Gene Ontology

	Molecular Function
GO:0003958	NADPH-hemoprotein reductase activity
GO:0003959	NADPH dehydrogenase activity
GO:0009055	electron transfer activity
GO:0010181	FMN binding
GO:0016491	oxidoreductase activity
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding

	Biological Process
GO:0006696	ergosterol biosynthetic process
GO:0016126	sterol biosynthetic process

	Cellular Component
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005741	mitochondrial outer membrane
GO:0005783	endoplasmic reticulum
GO:0005789	endoplasmic reticulum membrane
GO:0005829	cytosol
GO:0005886	plasma membrane

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2bn4, PDBe:2bn4, PDBj:2bn4
PDBsum	2bn4
PubMed	16407065
UniProt	P16603\|NCPR_YEAST NADPH--cytochrome P450 reductase (Gene Name=NCP1)

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