Structure of PDB 2bf4 Chain A

Receptor sequence

>2bf4A (length=645) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]

NRDIAQVVTENNKNYLVLYASQTGTAEDYAKKFSKELVAKFNLNVMCADV
ENYDFESLNDVPVIVSIFISTYGEGDFPDGAVNFEDFICNAEAGALSNLR
YNMFGLGNSTYEFFNGAAKKAEKHLSAAGAIRLGKLGEADDGAGTTDEDY
MAWKDSILEVLKDELHLDEQEAKFTSQFQYTVLNEITDSMSLGEPSAHYL
PSHQLNRNADGIQLGPFDLSQPYIAPIVKSRELFSSNDRNCIHSEFDLSG
SNIKYSTGDHLAVWPSNPLEKVEQFLSIFNLDPETIFDLKPLDPTVKVPF
PTPTTIGAAIKHYLEITGPVSRQLFSSLIQFAPNADVKEKLTLLSKDKDQ
FAVEITSKYFNIADALKYLSDGAKWDTVPMQFLVESVPQMTPRYYSISSS
SLSEKQTVHVTSIVENFPNPELPDAPPVVGVTTNLLRNIQLAQNNVNIAE
TNLPVHYDLNGPRKLFANYKLPVHVRRSNFRLPSNPSTPVIMIGPGTGVA
PFRGFIRERVAFLESQKKGGNNVSLGKHILFYGSRNTDDFLYQDEWPEYA
KKLDGSFEMVVAHSRLPNTKKVYVQDKLKDYEDQVFEMINNGAFIYVCGD
AKGMAKGVSTALVGILSRGKSITTDEATELIKMLKTSGRYQEDVW

3D structure

PDB

2bf4 A Second Fmn-Binding Site in Yeast Nadph-Cytochrome P450 Reductase Suggests a Mechanism of Electron Transfer by Diflavin Reductases.

Chain

Resolution

3.0 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	Y441 S442 C644 D689 W691
Catalytic site (residue number reindexed from 1)	Y395 S396 C598 D643 W645
Enzyme Commision number	1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	FAD	A	H306 G364 P365 Y405 F406 R439 Y440 Y441 S442 S458 I459 E461 F463 V474 V475 G476 V477 T478 T479 W691	H260 G318 P319 Y359 F360 R393 Y394 Y395 S396 S412 I413 E415 F417 V428 V429 G430 V431 T432 T433 W645
BS02	FMN	A	S67 Q68 T69 G70 T71 A72 S116 T117 Y118 G153 N154 Y157 F160 V690	S21 Q22 T23 G24 T25 A26 S70 T71 Y72 G107 N108 Y111 F114 V644
BS03	FMN	A	D74 Y75 K78 D187 D193 P365 V366 S367	D28 Y29 K32 D141 D147 P319 V320 S321
BS04	NAP	A	I459 E461 G542 S580 S610 R611 K617 Y619 Q621 D646 M650	I413 E415 G496 S534 S564 R565 K571 Y573 Q575 D600 M604

Gene Ontology

	Molecular Function
GO:0003958	NADPH-hemoprotein reductase activity
GO:0003959	NADPH dehydrogenase activity
GO:0009055	electron transfer activity
GO:0010181	FMN binding
GO:0016491	oxidoreductase activity
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding

	Biological Process
GO:0006696	ergosterol biosynthetic process
GO:0016126	sterol biosynthetic process

	Cellular Component
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005741	mitochondrial outer membrane
GO:0005783	endoplasmic reticulum
GO:0005789	endoplasmic reticulum membrane
GO:0005829	cytosol
GO:0005886	plasma membrane

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2bf4, PDBe:2bf4, PDBj:2bf4
PDBsum	2bf4
PubMed	16407065
UniProt	P16603\|NCPR_YEAST NADPH--cytochrome P450 reductase (Gene Name=NCP1)

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