Structure of PDB 2bcs Chain A

Receptor sequence

>2bcsA (length=324) Species: 9606 (Homo sapiens)

HNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACS
IPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQM
WYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQK
AAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSL
RQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFAC
ALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVL
PTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 2bcs Structural analysis of strand misalignment during DNA synthesis by a human DNA polymerase
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D176 D178 D239
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 R275 G278 Y279 P303 G304 G306 R308 M309	W23 R24 G27 Y28 P52 G53 G55 R57 M58
BS02	dna	A	W342 G343 A344 G345 K347 T348 D427 D429 D490 Y505 F506 T507 G508 A510 N513 R517	W91 G92 A93 G94 K96 T97 D176 D178 D239 Y254 F255 T256 G257 A259 N262 R266
BS03	dna	A	W274 V462 S463 Q464 Q471 K472 R514 R517 K521 L527 S528 E529 H530 H541 K544 P547	W23 V211 S212 Q213 Q220 K221 R263 R266 K270 L276 S277 E278 H279 H290 K293 P296
BS04	MG	A	D427 D429	D176 D178

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:2bcs, PDBe:2bcs, PDBj:2bcs
• PDBsum: 2bcs
• PubMed: 16439207
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)