Structure of PDB 2b0d Chain A

Receptor sequence
>2b0dA (length=236) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKEKI
KFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVLKTYNINELNEI
PKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSE
DEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB2b0d Non-cognate Enzyme-DNA Complex: Structural and Kinetic Analysis of EcoRV Endonuclease Bound to the EcoRI Recognition Site GAATTC
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A T37 N70 K92 T93 T94 Y95 T106 T111 S112 K119 S183 T186 N188 T36 N69 K91 T92 T93 Y94 T103 T108 S109 K116 S174 T177 N179 PDBbind-CN: Kd=900nM
BS02 dna A S183 G184 N185 T222 S223 R226 S174 G175 N176 T213 S214 R217 PDBbind-CN: Kd=900nM
BS03 CA A E45 D74 E44 D73
BS04 CA A D74 D90 D73 D89
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:2b0d, PDBe:2b0d, PDBj:2b0d
PDBsum2b0d
PubMed16236314
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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