Structure of PDB 2axn Chain A

Receptor sequence

>2axnA (length=451) Species: 9606 (Homo sapiens) [Search protein sequence]

LELTQSRVQKIWVPVDHRPSLPRSCGPNSPTVIVMVGLPARGKTYISKKL
TRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALA
ALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVC
DDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDK
CDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHG
ENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTI
QTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYY
RYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAE
EMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSNPLMRRNSS
S

3D structure

PDB

2axn Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy.

Chain

Resolution

2.1 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R252 H253 N259 S302 E322 H387
Catalytic site (residue number reindexed from 1)	R248 H249 N255 S298 E318 H383
Enzyme Commision number	2.7.1.105: 6-phosphofructo-2-kinase. 3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	F6P	A	I264 G265 E322 Y333 R347 K351 Y362 Q388 R392 T440	I260 G261 E318 Y329 R343 K347 Y358 Q384 R388 T436
BS02	EDT	A	P43 K47 N69 G71 R74 R75 F87 A125 T126 Y193	P39 K43 N65 G67 R70 R71 F83 A121 T122 Y189
BS03	ADP	A	P43 A44 R45 G46 K47 T48 Y49 S152 N163 E166 V167 K168 Y424	P39 A40 R41 G42 K43 T44 Y45 S148 N159 E162 V163 K164 Y420

Gene Ontology

	Molecular Function
GO:0003824	catalytic activity
GO:0003873	6-phosphofructo-2-kinase activity
GO:0004331	fructose-2,6-bisphosphate 2-phosphatase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0016787	hydrolase activity

	Biological Process
GO:0006000	fructose metabolic process
GO:0006003	fructose 2,6-bisphosphate metabolic process
GO:0006096	glycolytic process
GO:0006915	apoptotic process
GO:0010001	glial cell differentiation
GO:0016310	phosphorylation
GO:0046835	carbohydrate phosphorylation
GO:0061744	motor behavior

	Cellular Component
GO:0005654	nucleoplasm
GO:0005829	cytosol

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2axn, PDBe:2axn, PDBj:2axn
PDBsum	2axn
PubMed	16316985
UniProt	Q16875\|F263_HUMAN 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (Gene Name=PFKFB3)

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