Structure of PDB 2asp Chain A

Receptor sequence

>2aspA (length=361) Species: 9986 (Oryctolagus cuniculus) [Search protein sequence]

TTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRQKDSYVGDEAQSKRG
ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPK
ANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNV
PIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIK
EKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQ
PSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRM
QKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYD
EAGPSIVHRKC

3D structure

PDB

2asp Structures of microfilament destabilizing toxins bound to actin provide insight into toxin design and activity

Chain

Resolution

1.64 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.6.4.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	CA	A	Q263 S265	Q250 S252
BS02	ATP	A	G13 S14 G15 K18 G156 D157 G158 V159 G182 R210 K213 E214 G302 M305 Y306 K336	G9 S10 G11 K14 G143 D144 G145 V146 G169 R197 K200 E201 G289 M292 Y293 K323
BS03	RGC	A	Y143 A144 S145 G146 R147 P332 E334 I345 M355	Y130 A131 S132 G133 R134 P319 E321 I332 M342

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003785	actin monomer binding
GO:0005509	calcium ion binding
GO:0005515	protein binding
GO:0005523	tropomyosin binding
GO:0005524	ATP binding
GO:0016787	hydrolase activity
GO:0019904	protein domain specific binding
GO:0031013	troponin I binding
GO:0031432	titin binding
GO:0032036	myosin heavy chain binding
GO:0042802	identical protein binding
GO:0048306	calcium-dependent protein binding
GO:0140660	cytoskeletal motor activator activity

	Biological Process
GO:0010628	positive regulation of gene expression
GO:0030041	actin filament polymerization
GO:0030240	skeletal muscle thin filament assembly
GO:0048741	skeletal muscle fiber development
GO:0051017	actin filament bundle assembly
GO:0090131	mesenchyme migration

	Cellular Component
GO:0001725	stress fiber
GO:0005737	cytoplasm
GO:0005856	cytoskeleton
GO:0005865	striated muscle thin filament
GO:0005884	actin filament
GO:0030027	lamellipodium
GO:0030175	filopodium
GO:0031941	filamentous actin
GO:0032432	actin filament bundle
GO:0044297	cell body
GO:0098723	skeletal muscle myofibril

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:2asp, PDBe:2asp, PDBj:2asp
PDBsum	2asp
PubMed	16192358
UniProt	P68135\|ACTS_RABIT Actin, alpha skeletal muscle (Gene Name=ACTA1)

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