Structure of PDB 2aq4 Chain A

Receptor sequence

>2aq4A (length=428) Species: 4932 (Saccharomyces cerevisiae)

KRIVACDDPDFLTSYFAHSRLHHLSAWKANLKDKFLNENIHKYTKITDKD
TYIIFHIDFDCFFATVAYLCRSSSFSACDFKRDPIVVCHGTKNSDIASCN
YVARSYGIKNGMWVSQAEKMLPNGIKLISLPYTFEQFQLKSEAFYSTLKR
LNIFNLILPISIDEAVCVRIIPDTLNARLCEEIRQEIFQGTNGCTVSIGC
SDSLVLARLALKMAKPNGYNITFKSNLSEEFWSSFKLDDLPGVGHSTLSR
LESTFDSPHSLNDLRKRYTLDALKASVGSKLGMKIHLALQGQDDEESLKI
LYDPKEVLQRKSLSIDINWGIRFKNITQVDLFIERGCQYLLEKLNEINKT
TSQITLKLMRRCKDAPIEPPKYMGMGRCDSFSRSSRLGIPTNEFGIIATE
MKSLYRTLGCPPMELRGLALQFNKLVDV

3D structure

• PDB: 2aq4 Rev1 employs a novel mechanism of DNA synthesis using a protein template.
• Chain: A
• Resolution: 2.32 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	P551 G552 G554 S556 T557 S692 R693 S694 R696	P241 G242 G244 S246 T247 S382 R383 S384 R386
BS02	dna	A	I307 Y319 F320 H322 S323 R324 L325 H326 S329 K336 H393 G394 T395 K396 N397 S398 D399 S589 K590 E606 R620 K621 L623 S624 I625 D626 N628 W629 K681 Y682 G684 M685 G686	I3 Y15 F16 H18 S19 R20 L21 H22 S25 K32 H89 G90 T91 K92 N93 S94 D95 S279 K280 E296 R310 K311 L313 S314 I315 D316 N318 W319 K371 Y372 G374 M375 G376
BS03	MG	A	D362 D467 E468	D58 D163 E164
BS04	MG	A	D362 F363 D467	D58 F59 D163
BS05	DCP	A	R324 L328 D362 F363 D364 C365 F366 F367 A401 S402 Y405 R408 N414 D467 K525	R20 L24 D58 F59 D60 C61 F62 F63 A97 S98 Y101 R104 N110 D163 K215

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:2aq4, PDBe:2aq4, PDBj:2aq4
• PDBsum: 2aq4
• PubMed: 16195463
• UniProt: P12689|REV1_YEAST DNA repair protein REV1 (Gene Name=REV1)