Structure of PDB 2a4r Chain A

Receptor sequence

>2a4rA (length=181)

APITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCING
VCWTVYHGAGTRTIASPKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCG
SSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGHA
VGLFRAAVCTRGVAKAVDFIPVENLETTMRS

3D structure

• PDB: 2a4r Hepatitis C Virus NS3-4A serine protease inhibitors: Use of a P2-P1 cyclopropyl alanine combination for improved potency.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H57 D81 G137 S139
• Catalytic site (residue number reindexed from 1): H57 D81 G137 S139
• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.98: hepacivirin.
  3.4.22.-
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	T4 A5 Y6 A7 Q8 Q9 T10 R11 T19 S20 E30 E32 V33 Q34 I35 V36 S37 R62 T63 I64 A65 R109	T4 A5 Y6 A7 Q8 Q9 T10 R11 T19 S20 E30 E32 V33 Q34 I35 V36 S37 R62 T63 I64 A65 R109
BS02	peptide	A	T4 A5 Y6 A7 Q8	T4 A5 Y6 A7 Q8
BS03	ZN	A	C97 C99 C145	C97 C99 C145
BS04	BNH	A	Q41 T42 H57 R123 I132 K136 G137 S139 R155 A156 A157 C159	Q41 T42 H57 R123 I132 K136 G137 S139 R155 A156 A157 C159	PDBbind-CN: -logKd/Ki=7.82,Ki=15nM

Gene Ontology

Molecular Function

• GO:0008236 serine-type peptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019087 transformation of host cell by virus

External links

• PDB: RCSB:2a4r, PDBe:2a4r, PDBj:2a4r
• PDBsum: 2a4r
• PubMed: 16112862
• UniProt: P27958|POLG_HCV77 Genome polyprotein