Structure of PDB 1zrq Chain A

Receptor sequence
>1zrqA (length=281) Species: 562 (Escherichia coli) [Search protein sequence]
FFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNSIDRLSSLK
PKFVSVTYGADRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYW
NNGIRHIVALRGDLPEMYASDLVTLLKEVADFDISVAAYPEVHPEAKSAQ
ADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILP
VSNFKQAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKI
LSREGVKDFHFYTLNRAEMSYAICHTLGVRP
3D structure
PDB1zrq Structures of NADH and CH(3)-H(4)Folate Complexes of Escherichia coli Methylenetetrahydrofolate Reductase Reveal a Spartan Strategy for a Ping-Pong Reaction
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S26 E28 D120 F223 H273
Catalytic site (residue number reindexed from 1) S24 E26 D113 F210 H260
Enzyme Commision number 1.5.1.54: methylenetetrahydrofolate reductase (NADH).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD A T59 Y60 H88 T90 L117 R118 G119 D120 Y131 A132 Y152 H156 E158 A159 D165 N168 R171 K172 T57 Y58 H81 T83 L110 R111 G112 D113 Y118 A119 Y139 H143 E145 A146 D152 N155 R158 K159
BS02 NAI A F30 T59 Q183 F223 T227 L277 F28 T57 Q170 F210 T214 L264 MOAD: Ki=10uM
Gene Ontology
Molecular Function
GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity
GO:0016491 oxidoreductase activity
GO:0051087 protein-folding chaperone binding
GO:0071949 FAD binding
GO:0106312 methylenetetrahydrofolate reductase (NADH) activity
Biological Process
GO:0006555 methionine metabolic process
GO:0009086 methionine biosynthetic process
GO:0035999 tetrahydrofolate interconversion
Cellular Component
GO:0005829 cytosol
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1zrq, PDBe:1zrq, PDBj:1zrq
PDBsum1zrq
PubMed16114881
UniProtP0AEZ1|METF_ECOLI 5,10-methylenetetrahydrofolate reductase (Gene Name=metF)

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