Structure of PDB 1zgj Chain A

Receptor sequence

>1zgjA (length=354) Species: 3880 (Medicago truncatula)

SELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLSELASS
LKLHPSKVNILHRFLRLLTHNGFFAKTIVKGKEGDEEEEIAYSLTPPSKL
LISGKPTCLSSIVKGALHPSSLDMWSSSKKWFNEDKEQTLFECATGESFW
DFLNKDSESSTLSMFQDAMASDSRMFKLVLQENKRVFEGLESLVDVGGGT
GGVTKLIHEIFPHLKCTVFDQPQVVGNLTGNENLNFVGGDMFKSIPSADA
VLLKWVLHDWNDEQSLKILKNSKEAISHKGKDGKVIIIDISIDETSDDRG
LTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFSSYKITPISGFKSLI
EVYP

3D structure

• PDB: 1zgj Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H268 D269 D299 E330
• Catalytic site (residue number reindexed from 1): H258 D259 D289 E320
• Enzyme Commision number: 2.1.1.212: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase.
  2.1.1.46: isoflavone 4'-O-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	P1S	A	G125 A126 S131 F159 M174 F175 M179 Y318 M322	G115 A116 S121 F149 M164 F165 M169 Y308 M312
BS02	SAH	A	G207 V213 D230 Q231 D250 M251 F252 K264	G197 V203 D220 Q221 D240 M241 F242 K254

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0008171 O-methyltransferase activity
• GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
• GO:0030746 isoflavone 4'-O-methyltransferase activity
• GO:0046983 protein dimerization activity
• GO:0102670 2,7,4'-trihydroxyisoflavanone-4'-O-methyltransferase activity

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:1zgj, PDBe:1zgj, PDBj:1zgj
• PDBsum: 1zgj
• PubMed: 17172354
• UniProt: Q29U70|I4OMT_MEDTR Isoflavone 4'-O-methyltransferase (Gene Name=HI4'OMT)