Structure of PDB 1zca Chain A

Receptor sequence

>1zcaA (length=318) Species: 10090 (Mus musculus)

RLVKILLLGAGESGKSTFLKQMRIIHGREFDQKALLEFRDTIFDNILKGS
RVLVDARDKLGIPWQHSENEKHGMFLMAFENKAGLPVEPATFQLYVPALS
ALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYFPSKQDILLAR
KATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSS
EYDQVLMEDRRTNRLVESMNIFETIVNNKLFFNVSIILFLNKMDLLVEKV
KSVSIKKHFPDFKGDPHRLEDVQRYLVQCFDRKRRNRSKPLFHHFTTAID
TENIRFVFHAVKDTILQE

3D structure

• PDB: 1zca A new approach to producing functional G alpha subunits yields the activated and deactivated structures of G alpha(12/13) proteins.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E65 T70 R203 D225 Q229
• Catalytic site (residue number reindexed from 1): E12 T17 R150 D172 Q176

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	S69 T206	S16 T153
BS02	ALF	A	G64 K68 R203 A205 T206 G228 Q229	G11 K15 R150 A152 T153 G175 Q176
BS03	GDP	A	E65 S66 G67 K68 S69 T70 L201 R203 N294 K295 D297 L298 T350 A351 I352	E12 S13 G14 K15 S16 T17 L148 R150 N241 K242 D244 L245 T297 A298 I299

Gene Ontology

Molecular Function

• GO:0001664 G protein-coupled receptor binding
• GO:0003924 GTPase activity
• GO:0019001 guanyl nucleotide binding
• GO:0031683 G-protein beta/gamma-subunit complex binding

Biological Process

• GO:0007165 signal transduction
• GO:0007186 G protein-coupled receptor signaling pathway
• GO:0007266 Rho protein signal transduction

External links

• PDB: RCSB:1zca, PDBe:1zca, PDBj:1zca
• PDBsum: 1zca
• PubMed: 16388592
• UniProt: P27600|GNA12_MOUSE Guanine nucleotide-binding protein subunit alpha-12 (Gene Name=Gna12)