Structure of PDB 1yoe Chain A

Receptor sequence
>1yoeA (length=302) Species: 562 (Escherichia coli) [Search protein sequence]
GSALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNV
LRMLTLLNRTDIPVAGGAVKPLMRELIIAESGLDGPALPEPTFAPQNCTA
VELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAM
GLGNWTPAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTER
FRAIGNPVSTIVAELLDFFLEKWGFVGAPLHDPCTIAWLLKPELFTSVER
WVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGFVDLLADRLKF
YA
3D structure
PDB1yoe Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy
ChainA
Resolution1.78 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D10 D15 N39 L75 T124 F165 N166 H240 D241
Catalytic site (residue number reindexed from 1) D11 D16 N40 L76 T120 F161 N162 H231 D232
Enzyme Commision number 3.2.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 RIB A D14 N39 M150 N158 E164 F165 H240 D241 D15 N40 M146 N154 E160 F161 H231 D232
BS02 CA A D10 D15 T124 D241 D11 D16 T120 D232
Gene Ontology
Molecular Function
GO:0005509 calcium ion binding
GO:0005515 protein binding
GO:0008477 purine nucleosidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0016799 hydrolase activity, hydrolyzing N-glycosyl compounds
GO:0042802 identical protein binding
GO:0045437 uridine nucleosidase activity
GO:0050263 ribosylpyrimidine nucleosidase activity
Biological Process
GO:0006139 nucleobase-containing compound metabolic process
GO:0006152 purine nucleoside catabolic process
GO:0006206 pyrimidine nucleobase metabolic process
GO:0015949 nucleobase-containing small molecule interconversion
GO:0046133 pyrimidine ribonucleoside catabolic process
GO:0051289 protein homotetramerization
Cellular Component
GO:0005829 cytosol
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1yoe, PDBe:1yoe, PDBj:1yoe
PDBsum1yoe
PubMed
UniProtP41409|RIHA_ECOLI Pyrimidine-specific ribonucleoside hydrolase RihA (Gene Name=rihA)

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