Structure of PDB 1y7i Chain A

Receptor sequence

>1y7iA (length=258) Species: 4097 (Nicotiana tabacum)

EGKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEEL
RTLYDYTLPLMELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAV
FLAAFMPDSVHNSSFVLEQYNERTPAENWLDTQFLPYGSPEEPLTSMFFG
PKFLAHKLYQLCSPEDLALASSLVRPSSLFMEDLSKAKYFTDERFGSVKR
VYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAMLCEPQKLCASL
LEIAHKYN

3D structure

• PDB: 1y7i Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G12 S81 L82 D210 H238 M239
• Catalytic site (residue number reindexed from 1): G10 S79 L80 D208 H236 M237
• Enzyme Commision number: 3.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAL	A	A13 S81 L82 F107 Y122 W131 F151 H238	A11 S79 L80 F105 Y120 W129 F149 H236	MOAD: Kd=90nM
BS02	SAL	A	L132 H158 K159	L130 H156 K157	MOAD: Kd=90nM

Gene Ontology

Molecular Function

• GO:0016298 lipase activity
• GO:0016787 hydrolase activity
• GO:0080030 methyl indole-3-acetate esterase activity
• GO:0080031 methyl salicylate esterase activity
• GO:0080032 methyl jasmonate esterase activity

Biological Process

• GO:0009694 jasmonic acid metabolic process
• GO:0009696 salicylic acid metabolic process
• GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway
• GO:0045087 innate immune response

External links

• PDB: RCSB:1y7i, PDBe:1y7i, PDBj:1y7i
• PDBsum: 1y7i
• PubMed: 15668381
• UniProt: Q6RYA0|SABP2_TOBAC Salicylic acid-binding protein 2 (Gene Name=SABP2)