Structure of PDB 1xsp Chain A

Receptor sequence

>1xspA (length=327) Species: 9606 (Homo sapiens)

ATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQE
ACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKT
AQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQT
VQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLL
DSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSE
FACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGAKVGPG
RVLPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 1xsp A closed conformation for the Pol lambda catalytic cycle.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D179 D181 D242
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 S463 Q464 Q471 K472 R514 R517 K521 L527 E529 H530 H541	W26 V214 S215 Q216 Q223 K224 R266 R269 K273 L279 E281 H282 H293
BS02	dna	A	W342 G343 A344 G345 K347 T348 D427 D429 Y505 T507 G508 A510 N513 R517	W94 G95 A96 G97 K99 T100 D179 D181 Y257 T259 G260 A262 N265 R269
BS03	dna	A	W274 R275 G278 Y279 P303 G304 G306 R308 M309 K312	W26 R27 G30 Y31 P55 G56 G58 R60 M61 K64

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:1xsp, PDBe:1xsp, PDBj:1xsp
• PDBsum: 1xsp
• PubMed: 15608652
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)