Structure of PDB 1x9s Chain A

Receptor sequence

>1x9sA (length=673) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSDIEANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVD
GMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTF
WSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRK
LTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFCELDTREY
VAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKGAPVVDDEVLE
GVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGKIHGSV
NPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLDGITGKPWV
QAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQIAAELPTRDN
AKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAIAALRESIQ
QTLVEKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICKLWIIKTEE
MLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQVVIETAQEAMRWVG
DHWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

1x9s Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis.

Chain

Resolution

2.7 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T357 V363 V364 D365 D366 K394 R395 R429 A438 Q439 I440 P441 G442 V652 H653 D654	T337 V343 V344 D345 D346 K374 R375 R409 A418 Q419 I420 P421 G422 V621 H622 D623
BS02	dna	A	N335 S337 S338 H341 Q393 D403 K404 A425 V426 T431 H432 A433 F434 N436 Q439 R604 N611 Q615	N315 S317 S318 H321 Q373 D383 K384 A405 V406 T411 H412 A413 F414 N416 Q419 R573 N580 Q584

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:1x9s, PDBe:1x9s, PDBj:1x9s
PDBsum	1x9s
PubMed	15528277
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

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