Structure of PDB 1x8v Chain A

Receptor sequence
>1x8vA (length=429) Species: 1773 (Mycobacterium tuberculosis) [Search protein sequence]
SAVALPRVSGGHDEHGHLEEFRTDPIGLMQRVRDELGDVGTFQLAGKQVV
LLSGSHANEFFFRAGDDDLDQAKAYPFMTPIFGRRKEMLHNAALRGEQMK
GHAATIEDQVRRMIADWGEAGEIDLLDFFAELTIYTSSACLIGKKFRDQL
DGRFAKLYHELERGTDPLAYVDPYLPIESFRRRDEARNGLVALVADIMNG
RIANPRDMLDVLIAVKATPRFSADEITGMFISMMFAGHHTSSGTASWTLI
ELMRHRDAYAAVIDELDELYGDGRSVSFHALRQIPQLENVLKETLRLHPP
LIILMRVAKGEFEVQGHRIHEGDLVAASPAISNRIPEDFPDPHDFVPARY
EQPRQEDLLNRWTWIPFGAGRHRCVGAAFAIMQIKAIFSVLLREYEFEMA
QPPESYRNDHSKMVVQLAQPAAVRYRRRT
3D structure
PDB1x8v Estriol Bound and Ligand-free Structures of Sterol 14alpha-Demethylase.
ChainA
Resolution1.55 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T260 S261 F387 C394
Catalytic site (residue number reindexed from 1) T240 S241 F367 C374
Enzyme Commision number 1.14.15.36: sterol 14alpha-demethylase (ferredoxin).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A Q72 Y76 K97 H101 L105 A256 G257 T260 P320 L321 L324 R326 P386 F387 H392 C394 V395 G396 A400 Q71 Y75 K86 H90 L94 A236 G237 T240 P300 L301 L304 R306 P366 F367 H372 C374 V375 G376 A380
BS02 ESL A Y76 H259 L321 M433 Y75 H239 L301 M413 MOAD: Kd=100uM
BS03 ESL A M99 M225 L229 M88 M208 L212 MOAD: Kd=100uM
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0005515 protein binding
GO:0008398 sterol 14-demethylase activity
GO:0016491 oxidoreductase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
Biological Process
GO:0016125 sterol metabolic process
GO:0016126 sterol biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:1x8v, PDBe:1x8v, PDBj:1x8v
PDBsum1x8v
PubMed15530358
UniProtP9WPP9|CP51_MYCTU Sterol 14alpha-demethylase (Gene Name=cyp51)

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