Structure of PDB 1wd4 Chain A

Receptor sequence

>1wd4A (length=482)

MGPCDIYEAGDTPCVAAHSTTRALYSSFSGALYQLQRGSDDTTTTISPLT
AGGIADASAQDTFCANTTCLITIIYDQSGNGNHLTQAPPGGFDGPDTDGY
DNLASAIGAPVTLNGQKAYGVFMSPGTGYRNNEATGTATGDEAEGMYAVL
DGTHYNDACCFDYGNAETSSTDTGAGHMEAIYLGNSTTWGYGAGDGPWIM
VDMENNLFSGADEGYNSGDPSISYRFVTAAVKGGADKWAIRGANAASGSL
STYYSGARPDYSGYNPMSKEGAIILGIGGDNSNGAQGTFYEGVMTSGYPS
DDTENSVQENIVAAKYVVGSLVSGPSFTSGEVVSLRVTTPGYTTRYIAHT
DTTVNTQVVDDDSSTTLKEEASWTVVTGLANSQCFSFESVDTPGSYIRHY
NFELLLNANDGTKQFHEDATFCPQAALNGEGTSLRSWSYPTRYFRHYENV
LYAASNGGVQTFDSKTSFNNDVSFEIETAFAS

3D structure

• PDB: 1wd4 Crystal structure of a family 54 alpha-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose
• Chain: A
• Resolution: 2.07 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.55: non-reducing end alpha-L-arabinofuranosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AHR	A	M195 D219 E221 N222 N223 G295 G296 D297	M178 D202 E204 N205 N206 G278 G279 D280
BS02	AHR	A	H416 Y417 N418 F419 D435	H399 Y400 N401 F402 D418
BS03	AHR	A	Y359 H463 Y464 E465 N466 D488	Y342 H446 Y447 E448 N449 D471

Gene Ontology

Molecular Function

• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0046556 alpha-L-arabinofuranosidase activity

Biological Process

• GO:0019566 arabinose metabolic process
• GO:0031221 arabinan metabolic process
• GO:0031222 arabinan catabolic process
• GO:0045490 pectin catabolic process
• GO:0045493 xylan catabolic process
• GO:0046373 L-arabinose metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:1wd4, PDBe:1wd4, PDBj:1wd4
• PDBsum: 1wd4
• PubMed: 15292273
• UniProt: Q8NK89|ABFB_ASPKW Alpha-L-arabinofuranosidase B (Gene Name=abfB)