Structure of PDB 1w7k Chain A

Receptor sequence
>1w7kA (length=411) Species: 562 (Escherichia coli) [Search protein sequence]
TPQAASPLASWLSYLENLIDLGLERVSLVAARLGVLKPAPFVFTVAGTNG
KGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPESAHTASF
AEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIV
DADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTI
ADVAQEKGALLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAA
TALAALRASGLEVSENAIRDGIASAILPGRFQIVSESPRVIFDVAHNPHA
AEYLTGRMKALPKNGRVLAVIGMLHDKDIAGTLAWLKSVVDDWYCAPLEG
PRGATAEQLLEHLGNGKSFDSVAQAWDAAMADAKAEDTVLVCGSFHTVAH
VMEVIDARRSG
3D structure
PDB1w7k Escherichia Coli Folc Structure Reveals an Unexpected Dihydrofolate Binding Site Providing an Attractive Target for Anti-Microbial Therapy
ChainA
Resolution2.1 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 6.3.2.12: dihydrofolate synthase.
6.3.2.17: tetrahydrofolate synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADP A N58 G59 K60 G61 T62 N257 R289 D302 A304 H308 A309 Y312 N49 G50 K51 G52 T53 N248 R280 D293 A295 H299 A300 Y303
BS02 MG A S83 E146 S74 E137
Gene Ontology
Molecular Function
GO:0004326 tetrahydrofolylpolyglutamate synthase activity
GO:0005524 ATP binding
GO:0008841 dihydrofolate synthase activity
GO:0016874 ligase activity
GO:0016881 acid-amino acid ligase activity
GO:0046872 metal ion binding
GO:0097216 guanosine tetraphosphate binding
Biological Process
GO:0006730 one-carbon metabolic process
GO:0006761 dihydrofolate biosynthetic process
GO:0009058 biosynthetic process
GO:0009257 10-formyltetrahydrofolate biosynthetic process
GO:0009396 folic acid-containing compound biosynthetic process
GO:0046654 tetrahydrofolate biosynthetic process
GO:0046656 folic acid biosynthetic process
GO:0046901 tetrahydrofolylpolyglutamate biosynthetic process
Cellular Component
GO:0005737 cytoplasm

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:1w7k, PDBe:1w7k, PDBj:1w7k
PDBsum1w7k
PubMed15705579
UniProtP08192|FOLC_ECOLI Dihydrofolate synthase/folylpolyglutamate synthase (Gene Name=folC)

[Back to BioLiP]