Structure of PDB 1w7a Chain A

Receptor sequence

>1w7aA (length=791) Species: 562 (Escherichia coli)

SAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDAKRASQLL
DISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPAT
SKGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDIS
SGRFRLSEPADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPL
WEFEIDTARQQLNLQFGTRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTT
LPHIRSITMEREQDSIIMDAATRRNLEITQNLAGGAENTLASVLDCTVTP
MGSRMLKRWLHMPVRDTRVLLERQQTIGALQDFTAGLQPVLRQVGDLERI
LARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQALREKMGEFA
ELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERLE
VRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERY
IIPELKEYEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAE
LDVLVNLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPL
NLSPQRRMLIITGPNMGGKSTYMRQTALIALMAYIGSYVPAQKVEIGPID
RIFTRVGASTFMVEMTETANILHNATEYSLVLMDEIGRGTSTYDGLSLAW
ACAENLANKIKALTLFATHYFELTQLPEKMEGVANVHLDALEHGDTIAFM
HSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELESIS

3D structure

• PDB: 1w7a ATP Increases the Affinity between Muts ATPase Domains: Implications for ATP Hydrolysis and Conformational Changes
• Chain: A
• Resolution: 2.27 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	P12 M13 M33 G34 D35 F36 R58 S61 Q95 P99 P105 V106 R108	P11 M12 M32 G33 D34 F35 R57 S60 Q94 P98 P104 V105 R107	PDBbind-CN: Kd=7.5uM
BS02	dna	A	F36 E38 I53 S54 T56 M68 G70 P72 Y79 N468 L495 K496 R500	F35 E37 I52 S53 T55 M67 G69 P71 Y78 N467 L494 K495 R499	PDBbind-CN: Kd=7.5uM
BS03	ATP	A	L592 F596 I597 N616 M617 G619 K620 S621 T622 H760	L591 F595 I596 N615 M616 G618 K619 S620 T621 H751

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008301 DNA binding, bending
• GO:0016887 ATP hydrolysis activity
• GO:0030983 mismatched DNA binding
• GO:0032136 adenine/cytosine mispair binding
• GO:0042802 identical protein binding
• GO:0043531 ADP binding
• GO:0140664 ATP-dependent DNA damage sensor activity

Biological Process

• GO:0000018 regulation of DNA recombination
• GO:0006281 DNA repair
• GO:0006298 mismatch repair
• GO:0006974 DNA damage response

Cellular Component

• GO:0005829 cytosol
• GO:0032300 mismatch repair complex
• GO:1990710 MutS complex

External links

• PDB: RCSB:1w7a, PDBe:1w7a, PDBj:1w7a
• PDBsum: 1w7a
• PubMed: 15297450
• UniProt: P23909|MUTS_ECOLI DNA mismatch repair protein MutS (Gene Name=mutS)