Structure of PDB 1w78 Chain A

Receptor sequence

>1w78A (length=414) Species: 562 (Escherichia coli) [Search protein sequence]

TPQAASPLASWLSYLENLHSKTIDLGLERVSLVAARLGVLKPAPFVFTVA
GTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPESAH
TASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDA
TNIVDADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEM
PSTIADVAQEKGALLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQ
PNAATALAALRASGLEVSENAIRDGIASAILPGRFQIVSESPRVIFDVAH
NPHAAEYLTGRMKALPKNGRVLAVIGMLHDKDIAGTLAWLKSVVDDWYCA
PLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADAKAEDTVLVCGSFH
TVAHVMEVIDARRS

3D structure

PDB

1w78 Escherichia Coli Folc Structure Reveals an Unexpected Dihydrofolate Binding Site Providing an Attractive Target for Anti-Microbial Therapy

Chain

Resolution

1.82 Å

3D
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Enzymatic activity

Enzyme Commision number

6.3.2.12: dihydrofolate synthase.
6.3.2.17: tetrahydrofolate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	PD8	A	I28 D29 L30 G31 K60 P84 T122 F124 E125 G148 L153 D154 A155 H173	I23 D24 L25 G26 K55 P79 T117 F119 E120 G143 L148 D149 A150 H168
BS02	MG	A	S83 E146	S78 E141
BS03	ADP	A	N58 G59 K60 G61 T62 N257 R289 D302 V303 A304 H308 A309 Y312	N53 G54 K55 G56 T57 N252 R284 D297 V298 A299 H303 A304 Y307

Gene Ontology

	Molecular Function
GO:0004326	tetrahydrofolylpolyglutamate synthase activity
GO:0005524	ATP binding
GO:0008841	dihydrofolate synthase activity
GO:0016874	ligase activity
GO:0016881	acid-amino acid ligase activity
GO:0046872	metal ion binding
GO:0097216	guanosine tetraphosphate binding

	Biological Process
GO:0006730	one-carbon metabolic process
GO:0006761	dihydrofolate biosynthetic process
GO:0009058	biosynthetic process
GO:0009257	10-formyltetrahydrofolate biosynthetic process
GO:0009396	folic acid-containing compound biosynthetic process
GO:0046654	tetrahydrofolate biosynthetic process
GO:0046656	folic acid biosynthetic process
GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process

	Cellular Component
GO:0005737	cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1w78, PDBe:1w78, PDBj:1w78
PDBsum	1w78
PubMed	15705579
UniProt	P08192\|FOLC_ECOLI Dihydrofolate synthase/folylpolyglutamate synthase (Gene Name=folC)

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