Structure of PDB 1w6j Chain A

Receptor sequence
>1w6jA (length=727) Species: 9606 (Homo sapiens) [Search protein sequence]
CLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYA
LGLDTKNYFKDLPKAHTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLL
PGLLITCHVARIPLPAGYREEIVRYLRSVQLPDGGWGLHIEDKSTVFGTA
LNYVSLRILGVGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSW
EGLNTLFPEMWLFPDWAPAHPSTLWCHCRQVYLPMSYCYAVRLSAAEDPL
VQSLRQELYVEDFASIDWLAQRNNVAPDELYTPHSWLLRVVYALLNLYEH
HHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYVDGPAS
TAFQEHVSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRP
EFSSCLQKAHEFLRLSQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSD
CTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFATYET
KRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAE
IRETLTQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRD
GTACAEVSRACDFLLSRQMADGGWGEDFESCEERRYLQSAQSQIHNTCWA
MMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYT
SYRNIFPIWALGRFSQLYPERALAGHP
3D structure
PDB1w6j Insight Into Steroid Scaffold Formation from the Structure of Human Oxidosqualene Cyclase
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G107 L156 P190 H232 S339 W387 F444 D455 T457 A458 Y503 A536 W581 Y587 F696 C700 Y704
Catalytic site (residue number reindexed from 1) G102 L151 P185 H227 S334 W382 F439 D450 T452 A453 Y498 A531 W576 Y582 F691 C695 Y699
Enzyme Commision number 5.4.99.7: lanosterol synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 R71 A Y98 W192 W230 H232 C233 V236 W387 F444 D455 T502 I524 W581 F696 N697 Y93 W187 W225 H227 C228 V231 W382 F439 D450 T497 I519 W576 F691 N692 PDBbind-CN: -logKd/Ki=8.19,IC50=6.5nM
BindingDB: IC50=6.5nM
Gene Ontology
Molecular Function
GO:0000250 lanosterol synthase activity
GO:0005515 protein binding
GO:0016853 isomerase activity
GO:0016866 intramolecular transferase activity
Biological Process
GO:0006694 steroid biosynthetic process
GO:0006695 cholesterol biosynthetic process
GO:0008610 lipid biosynthetic process
GO:0016104 triterpenoid biosynthetic process
GO:0031647 regulation of protein stability
Cellular Component
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005811 lipid droplet
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1w6j, PDBe:1w6j, PDBj:1w6j
PDBsum1w6j
PubMed15525992
UniProtP48449|LSS_HUMAN Lanosterol synthase (Gene Name=LSS)

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