Structure of PDB 1w55 Chain A

Receptor sequence

>1w55A (length=369) Species: 197 (Campylobacter jejuni)

SEMSLIMLAAGNSTRFNTKVKKQFLRLGNDPLWLYATKNLSSFYPFKKIV
VTSSNITYMKKFTKNYEFIEGGDTRAESLKKALELIDSEFVMVSDVARVL
VSKNLFDRLIENLDKADCITPALKVADTTLFDNEALQREKIKLIQTPQIS
KTKLLKKALDQNLEFTDDSTAIAAMGGKIWFVEGEENARKLTFKEDLKKL
DLPTPSFEIFTGNGFDVHEFGENRPLLLAGVQIHPTMGLKAHSDGDVLAH
SLTDAILGAAGLGDIGELYPDTDMKFKNANSMELLKQAYDKVREIGFELI
NIDICVMAQSPKLKDFKQAMQSNIAHTLDLDEFRINVKATTTEKLGFIGR
KEGMAVLSSVNLKYFDWTR

3D structure

• PDB: 1w55 Hexameric Assembly of the Bifunctional Methylerythritol 2,4-Cyclodiphosphate Synthase and Protein-Protein Associations in the Deoxy-Xylulose-Dependent Pathway of Isoprenoid Precursor Biosynthesis
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.60: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase.
  4.6.1.12: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	C	A	D265 G267	D264 G266
BS02	C	A	L9 A10 A11 T75 R76 S79 D96 K191	L8 A9 A10 T74 R75 S78 D95 K190
BS03	GPP	A	F216 M308 G347 F348 R351	F215 M307 G346 F347 R350

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0008685 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0016829 lyase activity
• GO:0046872 metal ion binding
• GO:0050518 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
• GO:0070567 cytidylyltransferase activity

Biological Process

• GO:0008299 isoprenoid biosynthetic process
• GO:0016114 terpenoid biosynthetic process
• GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway

External links

• PDB: RCSB:1w55, PDBe:1w55, PDBj:1w55
• PDBsum: 1w55
• PubMed: 15466439
• UniProt: Q9PM68|ISPDF_CAMJE Bifunctional enzyme IspD/IspF (Gene Name=ispDF)