Structure of PDB 1w25 Chain A

Receptor sequence
>1w25A (length=454) Species: 190650 (Caulobacter vibrioides CB15) [Search protein sequence]
SARILVVDDIEANVRLLEAKLTAEYYEVSTAMDGPTALAMAARDLPDIIL
LDVMMPGMDGFTVCRKLKDDPTTRHIPVVLITALDGRGDRIQGLESGASD
FLTKPIDDVMLFARVRSLTRFKLVIDELRQREASGRRMGVIAGAAARLDG
LGGRVLIVDDNERQAQRVAAELGVEHRPVIESDPEKAKISAGGPVDLVIV
NAAAKNFDGLRFTAALRSEERTRQLPVLAMVDPDDRGRMVKALEIGVNDI
LSRPIDPQELSARVKTQIQRKRYTDYLRNNLDHSLELAVTDQLTGLHNRR
YMTGQLDSLVKRATLGGDPVSALLIDIDFFKKINDTFGHDIGDEVLREFA
LRLASNVRAIDLPCRYGGEEFVVIMPDTALADALRIAERIRMHVSGSPFT
VAHGREMLNVTISIGVSATAGEGDTPEALLKRADEGVYQAKASGRNAVVG
KAAH
3D structure
PDB1w25 Structural Basis of Activity and Allosteric Control of Diguanylate Cyclase
ChainA
Resolution2.7 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.65: diguanylate cyclase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D9 D10 D53 M55 D8 D9 D52 M54 Manual survey: Ki=0.5uM (15569936)
BS02 C2E A N335 D344 G369 E370 N334 D343 G368 E369 Manual survey: Ki=0.5uM (15569936)
PDBbind-CN: -logKd/Ki=6.30,Ki=0.5uM
BS03 C2E A R178 R359 D362 D383 I387 R390 R177 R358 D361 D382 I386 R389 Manual survey: Ki=0.5uM (15569936)
PDBbind-CN: -logKd/Ki=6.30,Ki=0.5uM
BS04 C2E A R148 V175 R178 S356 R359 A360 R390 R147 V174 R177 S355 R358 A359 R389 Manual survey: Ki=0.5uM (15569936)
PDBbind-CN: -logKd/Ki=6.30,Ki=0.5uM
Gene Ontology
Molecular Function
GO:0005525 GTP binding
GO:0016740 transferase activity
GO:0046872 metal ion binding
GO:0052621 diguanylate cyclase activity
Biological Process
GO:0000160 phosphorelay signal transduction system
GO:0030154 cell differentiation
GO:0043709 cell adhesion involved in single-species biofilm formation
GO:1902201 negative regulation of bacterial-type flagellum-dependent cell motility
Cellular Component
GO:0005737 cytoplasm
GO:0005886 plasma membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1w25, PDBe:1w25, PDBj:1w25
PDBsum1w25
PubMed15569936
UniProtB8GZM2|PLED_CAUVN Response regulator PleD (Gene Name=pleD)

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