Structure of PDB 1w1r Chain A

Receptor sequence

>1w1rA (length=479) Species: 4577 (Zea mays)

ALALDGKLRTDSNATAAASTDFGNITSALPAAVLYPSSTGDLVALLSAAN
STPGWPYTIAFRGRGHSLMGQAFAPGGVVVNMASLGDAAAPPRINVSADG
RYVDAGGEQVWIDVLRASLARGVAPRSWTDYLYLTVGGTLSNAGISGQAF
RHGPQISNVLEMDVITGHGEMVTCSKQLNADLFDAVLGGLGQFGVITRAR
IAVEPAPARARWVRFVYTDFAAFSADQERLTAPRSFGPMSYVEGSVFVNQ
SLATDLANTGFFTDADVARIVALAGERNATTVYSIEATLNYAAVDQELAS
VLGTLSYVEGFAFQRDVAYAAFLDRVHGEEVALNKLGLWRVPHPWLNMFV
PRSRIADFDRGVFKGILQGTDIVGPLIVYPLNKSMWDDGMSAATPSEDVF
YAVSLLFSSNDLARLQEQNRRILRFCDLAGIQYKTYLARHTDRSDWVRHF
GAAKWNRFVEMKNKYDPKRLLSPGQDIFN

3D structure

• PDB: 1w1r Structures of Michaelis and Product Complexes of Plant Cytokinin Dehydrogenase: Implications for Flavoenzyme Catalysis
• Chain: A
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H105 D169 E288
• Catalytic site (residue number reindexed from 1): H66 D130 E243
• Enzyme Commision number: 1.5.99.12: cytokinin dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	F61 F100 G102 R103 G104 H105 S106 Q110 T168 D169 Y170 L173 T174 G176 G177 S180 N181 G183 I184 V234 I235 W397 Y491 L492 S527	F22 F61 G63 R64 G65 H66 S67 Q71 T129 D130 Y131 L134 T135 G137 G138 S141 N142 G144 I145 V195 I196 W345 Y436 L437 S472
BS02	ZEA	A	D169 V378 W397 I429 S456 L458	D130 V326 W345 I377 S404 L406

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0019139 cytokinin dehydrogenase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0009690 cytokinin metabolic process

External links

• PDB: RCSB:1w1r, PDBe:1w1r, PDBj:1w1r
• PDBsum: 1w1r
• PubMed: 15321719
• UniProt: Q9T0N8|CKX1_MAIZE Cytokinin dehydrogenase 1 (Gene Name=CKX1)