Structure of PDB 1vrq Chain A

Receptor sequence

>1vrqA (length=963) Species: 31944 (Corynebacterium sp. U-96)

SKPQRLSAAQTAGARINRDEALTLTVDGQQLSAFRGDTVASAMLANGLRS
CGNSMYLDRPRGIFSAGVEEPNALITVGARHQADINESMLPATTVSVTDG
LNATLLSGLGVLDPSEDPAYYDHVHVHTDVLVVGAGPAGLAAAREASRSG
ARVMLLDERPEAGGTLREASGEQIDGIDAAQWIDAVTEELAAAEETTHLQ
RTTVFGSYDANYILAAQRRTVHLDGPSGQGVSRERIWHIRAKQVVLATAA
HERPIVFENNDRPGIMLAGSVRSYLNRFGVRAGSKIAVATTNDSVYPLVS
ELAASGGVVAVIDARQNISAAAAQAVTDGVTVLTGSVVANTEADASGELS
AVLVATLDEQRNLGEAQRFEADVLAVSGGFNPVVHLHSQRQGKLNWDTSI
HAFVPADAVANQHLAGALTGLLDTASALSTGAATGAAAASAAGFEKIAEV
PQALAVPAGETRPVWLVPSLSGDDAVHYKFHFVDLQRDQTVADVLRATGA
GMQSVEHIKRYTSISTANDQGKTSGVAAIGVIAAVLGIENPAQIGTTTFR
APYTPVSFAALAGRTRGELLDPARLTAMHPWHLAHGAKFEDVGQWKRPWY
YPQDGESMDEAVYRECKAVRDSVGMLDASTLGKIEIRGKDAAEFLNRMYT
NGYTKLKVGMGRYGVMCKADGMIFDDGVTLRLAEDRFLMHTTTGGAADVL
DWLEEWLQTEWPELDVTCTSVTEQLATVAVVGPRSRDVIAKLASSLDVSN
DAFKFMAFQDVTLDSGIEARISRISFSGELAFEIAIPAWHGLQVWEDVYA
AGQEFNITPYGTETMHVLRAEKGFIIVGQDTDGTVTPQDAGMEWVVSKLK
DFVGKRSFSREDNVREDRKHLVSVLPVDSSLRLAEGAALVAADAVASEGV
TPMEGWVTHAYNSPALGRTFGLALIKNGRNRIGEVLKTPVDGQLVDVQVS
DLVLFDPEGSRRD

3D structure

• PDB: 1vrq Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L349 L374 D676
• Catalytic site (residue number reindexed from 1): L349 L374 D676
• Enzyme Commision number: 1.5.3.24: sarcosine oxidase (5,10-methylenetetrahydrofolate-forming).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	G134 G136 P137 A138 D157 E158 R159 T165 V204 T248 A249 S294 F380 G416 L422 D423 T424	G134 G136 P137 A138 D157 E158 R159 T165 V204 T248 A249 S294 F380 G416 L422 D423 T424
BS02	FON	A	L631 Y663 G677 H690 I774 F776 E783 K822 F824	L631 Y663 G677 H690 I774 F776 E783 K822 F824
BS03	FMN	A	K509 R510 T516 Q520 T548 R550	K509 R510 T516 Q520 T548 R550

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008115 sarcosine oxidase activity
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0046653 tetrahydrofolate metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1vrq, PDBe:1vrq, PDBj:1vrq
• PDBsum: 1vrq
• PubMed: 15946648
• UniProt: Q50LF0|TSOXA_CORS9 Sarcosine oxidase subunit alpha (Gene Name=soxA)