Structure of PDB 1uwk Chain A

Receptor sequence

>1uwkA (length=554) Species: 303 (Pseudomonas putida)

NNKYRDVEIRAPRGNKLTAKSWLTEAPLRMLMNNLDPQVAENPKELVVYG
GIGRAARNWECYDKIVETLTRLEDDETLLVQSGKPVGVFKTHSNAPRVLI
ANSNLVPHWANWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETF
VEAGRQHYGGSLKGKWVLTAGLGGMGGAQPLAATLAGACSLNIESQQSRI
DFRLETRYVDEQATDLDDALVRIAKYTAEGKAISIALHGNAAEILPELVK
RGVRPDMVTDQTSAHDPLNGYLPAGWTWEQYRDRAQTEPAAVVKAAKQSM
AVHVQAMLDFQKQGVPTFDYGNNIRQMAKEEGVADAFDFPGFVPAYIRPL
FCRGVGPFRWAALSGEAEDIYKTDAKVKELIPDDAHLHRWLDMARERISF
QGLPARICWVGLGLRAKLGLAFNEMVRSGELSAPVVIGRDHLDSGSVSSP
NAETEAMRDGSDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGFSQHSG
MVIVCDGTDEAAERIARVLTNDPGTGVMRHADAGYDIAIDCAKEQGLDLP
MITG

3D structure

• PDB: 1uwk Structure and Action of Urocanase
• Chain: A
• Resolution: 1.19 Å

Enzymatic activity

• Enzyme Commision number: 4.2.1.49: urocanate hydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	Y52 G53 G54 I145 G176 G177 M178 G179 E197 S198 Q199 N243 A244 Q264 T265 S266 H268 Y274 L275 W281 Y323 G324 N325 F345 G493	Y49 G50 G51 I142 G173 G174 M175 G176 E194 S195 Q196 N240 A241 Q261 T262 S263 H265 Y271 L272 W278 Y320 G321 N322 F342 G490
BS02	URO	A	Y52 M132 T133 G144 M178 R362 D443	Y49 M129 T130 G141 M175 R359 D440

Gene Ontology

Molecular Function

• GO:0016153 urocanate hydratase activity
• GO:0016829 lyase activity

Biological Process

• GO:0006547 L-histidine metabolic process
• GO:0006548 L-histidine catabolic process
• GO:0019556 L-histidine catabolic process to glutamate and formamide
• GO:0019557 L-histidine catabolic process to glutamate and formate

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1uwk, PDBe:1uwk, PDBj:1uwk
• PDBsum: 1uwk
• PubMed: 15313616
• UniProt: P25080|HUTU_PSEPU Urocanate hydratase (Gene Name=hutU)