Structure of PDB 1ush Chain A

Receptor sequence

>1ushA (length=515) Species: 83333 (Escherichia coli K-12)

YEQDKTYKITVLHTNDHHGHFWRNEYGEYGLAAQKTLVDGIRKEVAAEGG
SVLLLSGGDINTGVPESDLQDAEPDFRGMNLVGYDAMAIGNHEFDNPLTV
LRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKRQDLKIAVIGLTTDDT
AKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHMGHYDNGE
HGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPC
KPDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKRVLY
TPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNM
GRLILAAQMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVY
ADMTGKEVIDYLTAVAQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPV
DPAKTYRMATLNFNATGGDGYPRLDNKPGYVNTGFIDAEVLKAYIQKSSP
LDVSVYEPKGEVSWQ

3D structure

• PDB: 1ush X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site.
• Chain: A
• Resolution: 1.73 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D41 H43 D84 N116 H117 D120 H217 H252 Q254 R375 R379 R410
• Catalytic site (residue number reindexed from 1): D16 H18 D59 N91 H92 D95 H192 H227 Q229 R340 R344 R375
• Enzyme Commision number: 3.1.3.5: 5'-nucleotidase.
  3.6.1.45: UDP-sugar diphosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	D41 H43 D84 Q254	D16 H18 D59 Q229
BS02	ZN	A	D84 N116 H217 H252	D59 N91 H192 H227
BS03	CO3	A	D84 N116 H117 H252 T518	D59 N91 H92 H227 T483

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0009166 nucleotide catabolic process

External links

• PDB: RCSB:1ush, PDBe:1ush, PDBj:1ush
• PDBsum: 1ush
• PubMed: 10331872
• UniProt: P07024|USHA_ECOLI Protein UshA (Gene Name=ushA)