Structure of PDB 1uon Chain A

Receptor sequence

>1uonA (length=1264) Species: 10891 (Reovirus sp.)

SSMILTQFGPFIESISGITDQSNDVFEDAAKAFSMFTRSDVYKALDEIPF
SDDAMLPIPPTIYTKPSHDSYYYIDALNRVRRKTYQGPDDVYVPNCSIVE
LLEPHETLTSYGRLSEAIENRAKDGDSQARIATTYGRIAESQARQIKAPL
EKFVLALLVAEAGGSLYDPVLQKYDEIPDLSHNCPLWCFREICRHISGPL
PDRAPYLYLSAGVFWLMSPRMTSAIPPLLSDLVNLAILQQTAGLDPSLVK
LGVQICLHAAASSSYSWFILKTKSIFPQNTLHSMYESLEGGYCPNLEWLE
PRSDYKFMYMGVMPLSAKYARSAPSNDKKARELGEKYGLSSVVGELRKRT
KTYVKHDFASVRYIRDAMACTSGIFLVRTPTETVLQEYTQSPEIKVPIPQ
KDWTGPIGEIRILKDTTSSIARYLYRTWYLAAARMAAQPRTWDPLFQAIM
RSQYVTARGGSGAALRESLYAINVSLPDFKGLPVKAATKIFQAAQLANLP
FSHTSVAILADTSMGLRNQVQRRPRSIMPLNVPQQQVSAPHTLTADYINY
HMNLSPTSGSAVIEKVIPLGVYASSPPNQSINIDISACDASITWDFFLSV
IMAAIHEGVASSSIGKPFMGVPASIVNDESVVGVRAARPISGMQNMIQHL
SKLYKRGFSYRVNDSFSPGNDFTHMTTTFPSGSTATSTEHTANNSTMMET
FLTVWGPEHTDDPDVLRLMKSLTIQRNYVCQGDDGLMIIDGTTAGKVNSE
TIQNDLELISKYGEEFGWKYDIAYDGTAEYLKLYFIFGCRIPNLSRHPIV
GKERANSSAEEPWPAILDQIMGVFFNGVHDGLQWQRWIRYSWALCCAFSR
QRTMIGESVGYLQYPMWSFVYWGLPLVKAFGSDPWIFSWYMPTGDLGMYS
WISLIRPLMTRWMVANGYVTDRCSTVFGNADYRRCFNELKLYQGYYMAQL
PRNPKKSGRAASREVREQFTQALSDYLMQNPELKSRVLRGRSEWEKYGAG
IIHNPPSLFDVPHKWYQGAQEAAIATREELAEMDETLMRARRHSYSSFSK
LLEAYLLVKWRMCEAREPSVDLRLPLCAGIDPLNSDPFLKMVSVGPMLQS
TRKYFAQTLFMAKTVSGLDVNAIDSALLRLRTLGADKKALTAQLLMVGLQ
ESEADALAGKIMLQDVNTVQLARVVNLAVPDTWMSLDFDSMFKHHVKLLP
KDGRHLNTDIPPRMGWLRAILRFLGAGMVMTATGVAVDIYLEDIHGGGRS
LGQRFMTWMRQEGR

3D structure

• PDB: 1uon Reovirus Polymerase Lambda3 Localized by Cryo-Electron Microscopy of Virions at a Resolution of 7.6 A
• Chain: A
• Resolution: 7.6 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	G560 S561 A562 K566 K783 H798 N827	G559 S560 A561 K565 K782 H797 N826
BS02	rna	A	Q454 T457 R459 G460 S462 K490 M515 R518 I528 P530 S559 E565 S682 G683 N807 D1182 M1185	Q453 T456 R458 G459 S461 K489 M514 R517 I527 P529 S558 E564 S681 G682 N806 D1181 M1184
BS03	CH1	A	R523 R524 R526 S587 D590 D734	R522 R523 R525 S586 D589 D733
BS04	CH1	A	K32 S35 M36 R851 Y862	K31 S34 M35 R850 Y861
BS05	CH1	A	Q852 R853 M855 D1035	Q851 R852 M854 D1034
BS06	MN	A	D585 I586 S587 D734	D584 I585 S586 D733
BS07	MN	A	D585 D734 D735	D584 D733 D734

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0019079 viral genome replication
• GO:0032774 RNA biosynthetic process

Cellular Component

• GO:0019013 viral nucleocapsid

External links

• PDB: RCSB:1uon, PDBe:1uon, PDBj:1uon
• PDBsum: 1uon
• PubMed: 14608373
• UniProt: Q8V1A3