Structure of PDB 1uob Chain A

Receptor sequence
>1uobA (length=282) Species: 1901 (Streptomyces clavuligerus) [Search protein sequence]
MDTTVPTFSLAELQQGLHQDEFRRCLRDKGLFYLTDCGLTDTELKSAKDL
VIDFFEHGSEAEKRAVTSPVPTMRRGFTGYSMCYSMGTADNLFPSGDFER
IWTQYFDRQYTASRAVAREVLRATGTEPDGGVEAFLDCEPLLRFRYFPQV
LRMAPHYDLSMVTLIQQTPCANGFVSLQAEVGGAFTDLPYRPDAVLVFCG
AIATLVTGGQVKAPRHHVAAPRRDQIAGSSRTSSVFFLRPNADFTFSVPL
ARECGFDVSLDGETATFQDWIGGNYVNIRRTS
3D structure
PDB1uob The Structural Basis of Cephalosporin Formation in a Mononuclear Ferrous Enzyme
ChainA
Resolution1.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R74
Catalytic site (residue number reindexed from 1) R74
Enzyme Commision number 1.14.20.1: deacetoxycephalosporin-C synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FE2 A H183 D185 H243 H156 D158 H216
BS02 PNN A M73 L158 R160 R162 M180 H183 I192 H243 V262 F264 M73 L141 R143 R145 M153 H156 I165 H216 V235 F237
BS03 AKG A M180 H183 L204 H243 R258 S260 M153 H156 L177 H216 R231 S233
Gene Ontology
Molecular Function
GO:0005506 iron ion binding
GO:0016491 oxidoreductase activity
GO:0016706 2-oxoglutarate-dependent dioxygenase activity
GO:0031418 L-ascorbic acid binding
GO:0050599 deacetoxycephalosporin-C synthase activity
Biological Process
GO:0009058 biosynthetic process
GO:0017000 antibiotic biosynthetic process

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Molecular Function
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Biological Process
External links
PDB RCSB:1uob, PDBe:1uob, PDBj:1uob
PDBsum1uob
PubMed14718929
UniProtP18548|CEFE_STRCL Deacetoxycephalosporin C synthase (Gene Name=cefE)

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