Structure of PDB 1ua7 Chain A

Receptor sequence

>1ua7A (length=422) Species: 1423 (Bacillus subtilis)

PSIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDK
SMSNWYWLYQPTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHT
TFDYAAISNEVKSIPNWTHGNTQIKNWSDRWDVTQNSLLGLYDWNTQNTQ
VQSYLKRFLERALNDGADGFRFDAAKHIELPDDGSYGSQFWPNITNTSAE
FQYGEILQDSASRDAAYANYMDVTASNYGHSIRSALKNRNLGVSNISHYA
SDVSADKLVTWVESHDTYANDDEESTWMSDDDIRLGWAVIASRSGSTPLF
FSRPEGGGNGVRFPGKSQIGDRGSALFEDQAITAVNRFHNVMAGQPEELS
NPQGNNQIFMNQRGSHGVVLANAGSSSVSINTATKLPDGRYDNKAGAGSF
QVNDGKLTGTINARSVAVLYPD

3D structure

• PDB: 1ua7 Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose
• Chain: A
• Resolution: 2.21 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.1: alpha-amylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	W58 Y59 Q63 L142 N273	W55 Y56 Q60 L139 N270
BS02	GLD	A	F105 L142	F102 L139
BS03	BGC	A	K179 D212	K176 D209
BS04	G6D	A	A177 H180 E208	A174 H177 E205
BS05	ACI	A	Q126 L142	Q123 L139
BS06	ACI	A	Y62 H102 D176 E208 H268 D269	Y59 H99 D173 E205 H265 D266
BS07	CA	A	N101 T137 D146 H180	N98 T134 D143 H177
BS08	CA	A	G169 D171	G166 D168
BS09	CA	A	E276 G313	E273 G310

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004556 alpha-amylase activity
• GO:0043169 cation binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:1ua7, PDBe:1ua7, PDBj:1ua7
• PDBsum: 1ua7
• PubMed: 14617662
• UniProt: P00691|AMY_BACSU Alpha-amylase (Gene Name=amyE)