Structure of PDB 1u3d Chain A

Receptor sequence

>1u3dA (length=485) Species: 3702 (Arabidopsis thaliana)

CSIVWFRRDLRVEDNPALAAAVRAGPVIALFVWAPEEEGHYHPGRVSRWW
LKNSLAQLDSSLRSLGTCLITKRSTDSVASLLDVVKSTGASQIFFNHLYD
PLSLVRDHRAKDVLTAQGIAVRSFNADLLYEPWEVTDELGRPFSMFAAFW
ERCLSMPYDPESPLLPPKKIISGDVSKCVADPLVFEDDSEKGSNALLARA
WSPGWSNGDKALTTFINGPLLEYSKNRRKADSATTSFLSPHLHFGEVSVR
KVFHLVRIKQVAWANEGNEAGEESVNLFLKSIGLREYSRYISFNHPYSHE
RPLLGHLKFFPWAVDENYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIR
VVVSSFFVKVLQLPWRWGMKYFWDTLLDADLESDALGWQYITGTLPDSRE
FDRIDNPQFEGYKFDPNGEYVRRWLPELSRLPTDWIHHPWNAPESVLQAA
GIELGSNYPLPIVGLDEAKARLHEALSQMWQLEAA

3D structure

• PDB: 1u3d Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thaliana.
• Chain: A
• Resolution: 2.45 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S293 L296 W324 D359 W377 W400
• Catalytic site (residue number reindexed from 1): S281 L284 W312 D347 W365 W388

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	N238 R239 T246	N226 R227 T234
BS02	MG	A	K241 S244 T246	K229 S232 T234
BS03	MG	A	N219 K222	N207 K210
BS04	FAD	A	Y235 T247 S248 F249 S251 L254 F290 S293 L296 R297 W356 D359 R362 S366 F384 D390 D392 S395 D396	Y223 T235 S236 F237 S239 L242 F278 S281 L284 R285 W344 D347 R350 S354 F372 D378 D380 S383 D384
BS05	ANP	A	R239 L296 D359 R360 V363 L398 Y402 D409	R227 L284 D347 R348 V351 L386 Y390 D397	MOAD: Kd=75uM
BS06	NDS	A	A212 W213 F249 H253	A200 W201 F237 H241

Gene Ontology

Molecular Function

• GO:0009882 blue light photoreceptor activity

Biological Process

• GO:0009785 blue light signaling pathway

External links

• PDB: RCSB:1u3d, PDBe:1u3d, PDBj:1u3d
• PDBsum: 1u3d
• PubMed: 15299148
• UniProt: Q43125|CRY1_ARATH Cryptochrome-1 (Gene Name=CRY1)