Structure of PDB 1tyt Chain A

Receptor sequence
>1tytA (length=487) Species: 5656 (Crithidia fasciculata) [Search protein sequence]
MSRAYDLVVIGAGSGGLEAGWNAASLHKKRVAVIDLQKHHGPPHYAALGG
TCVNVGCVPKKLMVTGANYMDTIRESAGFGWELDRESVRPNWKALIAAKN
KAVSGINDSYEGMFADTEGLTFHQGFGALQDNHTVLVRESADPNSAVLET
LDTEYILLATGSWPQHLGIEGDDLCITSNEAFYLDEAPKRALCVGGGYIS
IEFAGIFNAYKARGGQVDLAYRGDMILRGFDSELRKQLTEQLRANGINVR
THENPAKVTKNADGTRHVVFESGAEADYDVVMLAIGRVPRSQTLQLDKAG
VEVAKNGAIKVDAYSKTNVDNIYAIGDVTDRVMLTPVAINEGAAFVDTVF
ANKPRATDHTKVACAVFSIPPMGVCGYVEEDAAKKYDQVAVYESSFTPLM
HNISGSTYKKFMVRIVTNHADGEVLGVHMLGDSSPEIIQSVAICLKMGAK
ISDFYNTIGVHPTSAEELCSMRTPAYFYQKGKRVEKI
3D structure
PDB1tyt Structure of trypanothione reductase from Crithidia fasciculata at 2.6 A resolution; enzyme-NADP interactions at 2.8 A resolution.
ChainA
Resolution2.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S14 L48 C52 C57 K60 R85 E86 Y198 E202 I339 G459 H461 E466 E485 K486
Catalytic site (residue number reindexed from 1) S14 L48 C52 C57 K60 R85 E86 Y198 E202 I339 G459 H461 E466 E485 K486
Enzyme Commision number 1.8.1.12: trypanothione-disulfide reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD A I10 G11 G13 S14 D35 G50 T51 C52 G56 C57 K60 G127 T160 G161 Y198 I199 R287 R290 G326 D327 L334 T335 I10 G11 G13 S14 D35 G50 T51 C52 G56 C57 K60 G127 T160 G161 Y198 I199 R287 R290 G326 D327 L334 T335
Gene Ontology
Molecular Function
GO:0004362 glutathione-disulfide reductase (NADPH) activity
GO:0015036 disulfide oxidoreductase activity
GO:0015042 trypanothione-disulfide reductase (NADPH) activity
GO:0016491 oxidoreductase activity
GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
GO:0050660 flavin adenine dinucleotide binding
Biological Process
GO:0006749 glutathione metabolic process
GO:0034599 cellular response to oxidative stress
GO:0045454 cell redox homeostasis
GO:0098869 cellular oxidant detoxification
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1tyt, PDBe:1tyt, PDBj:1tyt
PDBsum1tyt
PubMed15299452
UniProtP39040|TYTR_CRIFA Trypanothione reductase (Gene Name=TPR)

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