Structure of PDB 1trk Chain A

Receptor sequence

>1trkA (length=678) Species: 4932 (Saccharomyces cerevisiae)

QFTDIDKLAVSTIRILAVDTVSKANSGHPGAPLGMAPAAHVLWSQMRMNP
TNPDWINRDRFVLSNGHAVALLYSMLHLTGYDLSIEDLKQFRQLGSRTPG
HPEFELPGVEVTTGPLGQGISNAVGMAMAQANLAATYNKPGFTLSDNYTY
VFLGDGCLQEGISSEASSLAGHLKLGNLIAIYDDNKITIDGATSISFDED
VAKRYEAYGWEVLYVENGNEDLAGIAKAIAQAKLSKDKPTLIKMTTTIGY
GSLHAGSHSVHGAPLKADDVKQLKSKFGFNPDKSFVVPQEVYDHYQKTIL
KPGVEANNKWNKLFSEYQKKFPELGAELARRLSGQLPANWESKLPTYTAK
DSAVATRKLSETVLEDVYNQLPELIGGSADLTPSNLTRWKEALDFQPPSS
GSGNYSGRYIRYGIREHAMGAIMNGISAFGANYKPYGGTFLNFVSYAAGA
VRLSALSGHPVIWVATHDSIGVGEDGPTHQPIETLAHFRSLPNIQVWRPA
DGNEVSAAYKNSLESKHTPSIIALSRQNLPQLEGSSIESASKGGYVLQDV
ANPDIILVATGSEVSLSVEAAKTLAAKNIKARVVSLPDFFTFDKQPLEYR
LSVLPDNVPIMSVEVLATTCWGKYAHQSFGIDRFGASGKAPEVFKFFGFT
PEGVAERAQKTIAFYKGDKLISPLKKAF

3D structure

• PDB: 1trk Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 A resolution.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H30 I250 H263 E418 H481
• Catalytic site (residue number reindexed from 1): H28 I248 H261 E416 H479
• Enzyme Commision number: 2.2.1.1: transketolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D157 N187 I189	D155 N185 I187
BS02	TPP	A	H69 L118 D157 G158 N187 I189 I191 H263	H67 L116 D155 G156 N185 I187 I189 H261
BS03	TPP	A	D382 E418 F445 Y448	D380 E416 F443 Y446

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004802 transketolase activity
• GO:0005515 protein binding
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006098 pentose-phosphate shunt

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:1trk, PDBe:1trk, PDBj:1trk
• PDBsum: 1trk
• PubMed: 8176731
• UniProt: P23254|TKT1_YEAST Transketolase 1 (Gene Name=TKL1)