Structure of PDB 1tk5 Chain A

Receptor sequence

>1tk5A (length=681) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSDIEANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVD
GMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTF
WSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRK
LTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFKKPKAQRE
GRCELDTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKG
APVVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYV
AEDGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHH
LDGITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQ
IAAELPTRDNAKTFIYGFLYGAIGQIVGAGKERGKELKKKFLENTPAIAA
LRESIQQTLVESVKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICK
LWIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQVVIETA
QEAMRWVGDHWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

1tk5 Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase.

Chain

Resolution

2.2 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T357 K359 V363 D365 D366 K394 R429 A438 Q439 P441 G442 H653	T347 K349 V353 D355 D356 K384 R419 A428 Q429 P431 G432 H630
BS02	dna	A	N335 S338 G397 K404 A425 V426 H432 A433 F434 N436 Q439 Y530 R604 N611 Q615	N325 S328 G387 K394 A415 V416 H422 A423 F424 N426 Q429 Y520 R581 N588 Q592

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:1tk5, PDBe:1tk5, PDBj:1tk5
PDBsum	1tk5
PubMed	15297882
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

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