Structure of PDB 1tk0 Chain A

Receptor sequence

>1tk0A (length=686) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSDIEANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVD
GMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTF
WSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRK
LTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFKKPEGREP
CELDTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKGAP
VVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAE
DGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLD
GITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQIA
AELPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAI
AALRESIQQTLVESSQQVKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAG
ALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQV
VIETAQEAMRWVGDHWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

1tk0 Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase.

Chain

Resolution

2.3 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	R111 T357 K359 V363 D365 D366 K394 R429 A438 Q439 I440 G442 V652 H653 H704	R111 T345 K347 V351 D353 D354 K382 R417 A426 Q427 I428 G430 V634 H635 H686
BS02	dna	A	S337 S338 G402 K404 A425 V426 T431 H432 A433 F434 N436 Q439 G527 Y530 A532 G533 K536 R604 H607 Q615	S325 S326 G390 K392 A413 V414 T419 H420 A421 F422 N424 Q427 G515 Y518 A520 G521 K524 R586 H589 Q597
BS03	MG	A	D475 A476 D654	D463 A464 D636
BS04	MG	A	D475 D654	D463 D636
BS05	DCT	A	R429 D475 A476 S477 G478 L479 E480 H506 R518 K522 Y526 D654	R417 D463 A464 S465 G466 L467 E468 H494 R506 K510 Y514 D636

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:1tk0, PDBe:1tk0, PDBj:1tk0
PDBsum	1tk0
PubMed	15297882
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

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