Structure of PDB 1t3i Chain A

Receptor sequence

>1t3iA (length=406) Species: 1143 (Synechocystis sp.)

PSLAATVRQDFPILNQEINGHPLVYLDNAATSQKPRAVLEKLMHYYENDN
ANVGAHQLSVRATDAYEAVRNKVAKFINARSPREIVYTRNATEAINLVAY
SWGMNNLKAGDEIITTVMEHHSNLVPWQMVAAKTGAVLKFVQLDEQESFD
LEHFKTLLSEKTKLVTVVHISNTLGCVNPAEEIAQLAHQAGAKVLVDACQ
SAPHYPLDVQLIDCDWLVASGHKMCAPTGIGFLYGKEEILEAMPPFFGGG
EMIAEVFFDHFTTGELPHKFEAGTPAIAEAIALGAAVDYLTDLGMENIHN
YEVELTHYLWQGLGQIPQLRLYGPNPKHGDRAALASFNVAGLHASDVATM
VDQDGIAIRSGHHCTQPLHRLFDASGSARASLYFYNTKEEIDLFLQSLQA
TIRFFS

3D structure

• PDB: 1t3i Kinetic and structural characterization of Slr0077/SufS, the essential cysteine desulfurase from Synechocystis sp. PCC 6803.
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H128 D205 C207 Q208 K231 R367 C372
• Catalytic site (residue number reindexed from 1): H120 D197 C199 Q200 K223 R359 C364
• Enzyme Commision number: 2.8.1.7: cysteine desulfurase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TU4	A	L151 D152 E153 E155 Y330 H377 F380	L143 D144 E145 E147 Y322 H369 F372
BS02	PLP	A	N98 T100 H128 N180 D205 C207 Q208 S228 H230 K231	N90 T92 H120 N172 D197 C199 Q200 S220 H222 K223
BS03	PLP	A	G281 T282	G273 T274

Gene Ontology

Molecular Function

• GO:0016740 transferase activity
• GO:0030170 pyridoxal phosphate binding
• GO:0031071 cysteine desulfurase activity

Biological Process

• GO:0006534 cysteine metabolic process

External links

• PDB: RCSB:1t3i, PDBe:1t3i, PDBj:1t3i
• PDBsum: 1t3i
• PubMed: 15379559
• UniProt: Q55793|CSD_SYNY3 Probable cysteine desulfurase (Gene Name=csd)