Structure of PDB 1t0s Chain A

Receptor sequence

>1t0sA (length=491) Species: 316 (Stutzerimonas stutzeri)

SMLKREDWYDLTRTTNWTPKYVTENELFPEEMSGARGISMEAWEKYDEPY
KITYPEYVSIQREKDSGAYSIKAALERDGFVDRADPGWVSTMQLHFGAIA
LEEYAASTAEARMARFAKAPGNRNMATFGMMDENRHGQIQLYFPYANVKR
SRKWDWAHKAIHTNEWAAIAARSFFDDMMMTRDSVAVSIMLTFAFETGFT
NMQFLGLAADAAEAGDHTFASLISSIQTDESRHAQQGGPSLKILVENGKK
DEAQQMVDVAIWRSWKLFSVLTGPIMDYYTPLESRNQSFKEFMLEWIVAQ
FERQLLDLGLDKPWYWDQFMQDLDETHHGMHLGVWYWRPTVWWDPAAGVS
PEEREWLEEKYPGWNDTWGQCWDVITDNLVNGKPELTVPETLPTICNMCN
LPIAHTPGNKWNVKDYQLEYEGRLYHFGSEADRWCFQIDPERYKNHTNLV
DRFLKGEIQPADLAGALMYMSLEPGVMGDDAHDYEWVKAYQ

3D structure

• PDB: 1t0s Crystal Structure of the Toluene/o-Xylene Monooxygenase Hydroxylase from Pseudomonas stutzeri OX1: INSIGHT INTO THE SUBSTRATE SPECIFICITY, SUBSTRATE CHANNELING, AND ACTIVE SITE TUNING OF MULTICOMPONENT MONOOXYGENASES.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E104 E134 H137 E197 E231 H234
• Catalytic site (residue number reindexed from 1): E103 E133 H136 E196 E230 H233

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE	A	E134 E197 E231 H234	E133 E196 E230 H233
BS02	FE	A	E104 E134 H137	E103 E133 H136
BS03	MCR	A	E104 E134 E197	E103 E133 E196
BS04	BML	A	H96 I100 Q204 L272 T273	H95 I99 Q203 L271 T272
BS05	BML	A	G207 L208 D211 T273 M277	G206 L207 D210 T272 M276
BS06	BML	A	W89 D211 T281 P282	W88 D210 T280 P281
BS07	BML	A	E391 L393 D463 L464	E390 L392 D462 L463
BS08	BML	A	M3 P56	M2 P55

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

External links

• PDB: RCSB:1t0s, PDBe:1t0s, PDBj:1t0s
• PDBsum: 1t0s
• PubMed: 15096510
• UniProt: O87798