Structure of PDB 1sx8 Chain A

Receptor sequence
>1sx8A (length=241) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIATTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVKSSLKTYNIN
ELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKG
IFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1sx8 DNA Cleavage by EcoRV Endonuclease: Two Metal Ions in Three Metal Ion Binding Sites
ChainA
Resolution2.15 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A T37 S41 N70 A92 T93 T94 Y95 T106 G109 K119 N120 S183 T186 N188 T36 S40 N69 A91 T92 T93 Y94 T105 G108 K118 N119 S179 T182 N184
BS02 dna A K38 Q69 N70 L180 S183 G184 N185 S223 K37 Q68 N69 L176 S179 G180 N181 S219
BS03 MN A D74 D90 D73 D89
BS04 MN A E45 D74 E44 D73
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1sx8, PDBe:1sx8, PDBj:1sx8
PDBsum1sx8
PubMed15170321
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

[Back to BioLiP]