Structure of PDB 1sl1 Chain A

Receptor sequence

>1sl1A (length=678) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSDIEANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVD
GMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTF
WSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRK
LTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFKKPEPCEL
DTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKGAPVVD
DEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGK
IHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLDGIT
GKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQIAAEL
PTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAIAAL
RESIQQTLVEKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICKLWI
IKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQVVIETAQEA
MRWVGDHWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

1sl1 Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7.

Chain

Resolution

2.2 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R339 T357 K359 V363 V364 D365 D366 K394 A438 Q439 I440 P441 G442 S445 Y526 H704	R324 T342 K344 V348 V349 D350 D351 K379 A423 Q424 I425 P426 G427 S430 Y511 H678
BS02	dna	A	S337 S338 G402 D403 K404 H432 A433 F434 N436 Q439 Y526 G527 A532 G533	S322 S323 G387 D388 K389 H417 A418 F419 N421 Q424 Y511 G512 A517 G518

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:1sl1, PDBe:1sl1, PDBj:1sl1
PDBsum	1sl1
PubMed	15235589
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

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