Structure of PDB 1sks Chain A

Receptor sequence

>1sksA (length=681) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSDIEANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVD
GMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTF
WSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRK
LTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFKKPKNREP
CELDTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKGAP
VVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAE
DGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLD
GITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQIA
AELPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAI
AALRESIQQTLVEKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICK
LWIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQVVIETA
QEAMRWVGDHWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

1sks Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7.

Chain

Resolution

2.3 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R111 R339 T357 K359 V363 D365 D366 K394 R429 A438 Q439 I440 G442 H704	R111 R327 T345 K347 V351 D353 D354 K382 R417 A426 Q427 I428 G430 H681
BS02	dna	A	N335 S337 S338 G402 K404 A433 F434 N436 Q439 Y530 G533 R604	N323 S325 S326 G390 K392 A421 F422 N424 Q427 Y518 G521 R581

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:1sks, PDBe:1sks, PDBj:1sks
PDBsum	1sks
PubMed	15235589
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

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