Structure of PDB 1seu Chain A

Receptor sequence

>1seuA (length=565) Species: 9606 (Homo sapiens)

QKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLS
PKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKC
DFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERI
ANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHK
WKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRL
KKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETAD
TVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKN
LQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQ
QLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTK
IDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMK
LEVQATDREENKQIALGTSKLSYLDPRITVAWCKKWGVPIEKIYNKTQRE
KFAWAIDMADEDYEF

3D structure

• PDB: 1seu Structures of three classes of anticancer agents bound to the human topoisomerase I-DNA covalent complex
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	V410 T411 W412 I424 Y426 K436 K439 K443 K532 K587 S722 Y723	V210 T211 W212 I224 Y226 K236 K239 K243 K332 K387 S522 Y523	PDBbind-CN: IC50=0.03uM
BS02	dna	A	R316 K328 R634 G717 T718	R116 K128 R434 G517 T518	PDBbind-CN: IC50=0.03uM
BS03	dna	A	F361 R362 G363 R364 H367 R488 A489 N491 K493 T501 K532 D533 T585 A586 K587 L647 K746	F161 R162 G163 R164 H167 R288 A289 N291 K293 T301 K332 D333 T385 A386 K387 L447 K546	PDBbind-CN: IC50=0.03uM
BS04	SA3	A	N352 R364	N152 R164	BindingDB: IC50=2300nM

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1seu, PDBe:1seu, PDBj:1seu
• PDBsum: 1seu
• PubMed: 15801827
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)