Structure of PDB 1sc7 Chain A

Receptor sequence

>1sc7A (length=567) Species: 9606 (Homo sapiens)

EEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMK
LSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLS
KCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKE
RIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPG
HKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETAR
RLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGET
ADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVF
KNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITL
QQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQ
TKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQL
MKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQ
REKFAWAIDMADEDYEF

3D structure

• PDB: 1sc7 Structures of three classes of anticancer agents bound to the human topoisomerase I-DNA covalent complex
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	V410 T411 W412 I424 Y426 M428 K436 K439 K443 N722 Y723	V212 T213 W214 I226 Y228 M230 K238 K241 K245 N524 Y525	PDBbind-CN: IC50=4.9uM
BS02	dna	A	R634 K638 G717 T718	R436 K440 G519 T520	PDBbind-CN: IC50=4.9uM
BS03	dna	A	F361 R362 G363 R364 H367 K374 R488 A489 N491 K493 T501 K532 D533 N574 T585 A586 K587 K650	F163 R164 G165 R166 H169 K176 R290 A291 N293 K295 T303 K334 D335 N376 T387 A388 K389 K452	PDBbind-CN: IC50=4.9uM
BS04	M38	A	A351 N352	A153 N154

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1sc7, PDBe:1sc7, PDBj:1sc7
• PDBsum: 1sc7
• PubMed: 15801827
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)