Structure of PDB 1sa3 Chain A

Receptor sequence
>1sa3A (length=262) Species: 479 (Moraxella sp.) [Search protein sequence]
MRTELLSKLYDDFGIDQLPHTQHGVTSDRLGKLYEKYILDIFKDIESLKK
YNTNAFPQEKDISSKLLKALNLDLDNIIDVSSSDTDLGRTIAGGSPKTDA
TIRFTFHNQSSRLVPLNIKHSSKKKVSIAEYDVETICTGVGISDGELKEL
IRKHQNDQSAKLFTPVQKQRLTELLEPYRERFIRWCVTLRAEKSEGNILH
PDLLIRFQVIDREYVDVTIKNIDDYVSDRIAEGSKARKPGFGTGLNWTYA
SGSKAKKMQFKG
3D structure
PDB1sa3 An Asymmetric Complex of Restriction Endonuclease MspI on Its Palindromic DNA Recognition Site.
ChainA
Resolution1.95 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A K119 H120 S121 S122 K123 S127 E130 G252 S253 K254 K257 Q259 K261 K119 H120 S121 S122 K123 S127 E130 G252 S253 K254 K257 Q259 K261
BS02 dna A S159 A160 K161 T248 Y249 S251 S159 A160 K161 T248 Y249 S251
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1sa3, PDBe:1sa3, PDBj:1sa3
PDBsum1sa3
PubMed15341737
UniProtP11405|T2M1_MORSP Type II restriction enzyme MspI (Gene Name=mspIR)

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