Structure of PDB 1s5n Chain A

Receptor sequence

>1s5nA (length=386) Species: 1963 (Streptomyces olivochromogenes)

SYQPTPEDRFTFGLWTVGWQGRDPFGDATRPALDPVETVQRLAELGAHGV
TFHDDDLIPFGSSDTERESHIKRFRQALDATGMTVPMATTNLFTHPVFKD
GGFTANDRDVRRYALRKTIRNIDLAVELGAKTYVAWGGREGAESGAAKDV
RVALDRMKEAFDLLGEYVTSQGYDIRFAIEPKPNEPRGDILLPTVGHALA
FIERLERPELYGVNPEVGHEQMAGLNFPHGIAQALWAGKLFHIDLNGQSG
IKYDQDLRFGAGDLRAAFWLVDLLESAGYEGPRHFDFKPPRTEDIDGVWA
SAAGCMRNYLILKERAAAFRADPEVQEALRASRLDELAQPTAADGVQELL
ADRTAFEDFDVDAAAARGMAFERLDQLAMDHLLGAR

3D structure

• PDB: 1s5n Xylose isomerase in substrate and inhibitor michaelis States: atomic resolution studies of a metal-mediated hydride shift(,).
• Chain: A
• Resolution: 0.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 D56 M87 E180 K182 E216 H219 D244 D254 D256 D286
• Catalytic site (residue number reindexed from 1): H53 D56 M87 E180 K182 E216 H219 D244 D254 D256 D286
• Enzyme Commision number: 5.3.1.5: xylose isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	XYL	A	H53 W136 E180 K182 E216 H219 D286	H53 W136 E180 K182 E216 H219 D286
BS02	MN	A	E180 E216 D244 D286	E180 E216 D244 D286
BS03	MN	A	E216 H219 D254 D256	E216 H219 D254 D256

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0009045 xylose isomerase activity
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0042732 D-xylose metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1s5n, PDBe:1s5n, PDBj:1s5n
• PDBsum: 1s5n
• PubMed: 15157080
• UniProt: P15587|XYLA_STROL Xylose isomerase (Gene Name=xylA)