Structure of PDB 1rrj Chain A

Receptor sequence

>1rrjA (length=565) Species: 9606 (Homo sapiens)

QKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLS
PKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKC
DFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERI
ANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHK
WKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRL
KKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETAD
TVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKN
LQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQ
QLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTK
IDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMK
LEVQATDREENKQIALGTSKLSYLDPRITVAWCKKWGVPIEKIYNKTQRE
KFAWAIDMADEDYEF

3D structure

• PDB: 1rrj Mechanisms of camptothecin resistance by human topoisomerase I mutations
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T313 R316 K324 V410 T411 W412 I424 Y426 M428 K439 K443 K587 R634 K650 A715 G717 T718 L721 S722 Y723	T113 R116 K124 V210 T211 W212 I224 Y226 M228 K239 K243 K387 R434 K450 A515 G517 T518 L521 S522 Y523
BS02	dna	A	E356 F361 R362 G363 R364 H367 K374 R488 A489 G490 N491 K493 T501 K532 D533 N574 T585 A586 K587 F640 N646	E156 F161 R162 G163 R164 H167 K174 R288 A289 G290 N291 K293 T301 K332 D333 N374 T385 A386 K387 F440 N446
BS03	TTG	A	N352 K532 D533 T718	N152 K332 D333 T518
BS04	TTC	A	D533 T718	D333 T518	BindingDB: EC50=54nM,IC50=5250nM,Kd=26.5nM

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1rrj, PDBe:1rrj, PDBj:1rrj
• PDBsum: 1rrj
• PubMed: 15165849
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)