Structure of PDB 1rq5 Chain A

Receptor sequence

>1rq5A (length=602) Species: 1515 (Acetivibrio thermocellus)

ILPQPDVRVNQVGYLPEGKKVATVVCNSTQPVKWQLKNAAGVVVLEGYTE
PKGLDKDSQDYVHWLDFSDFATEGIGYYFELPTVNSPTNYSHPFDIRKDI
YTQMKYDALAFFYHKRSGIPIEMPYAGGEQWTRPAGHIGIEPNKGDTNVP
TWPQDDEYAGIPQKNYTKDVTGGWYDAGDHGKYVVNGGIAVWTLMNMYER
AKIRGLDNWGPYRDGGMNIPEQNNGYPDILDEARWEIEFFKKMQVTEKED
PSIAGMVHHKIHDFRWTALGMLPHEDPQPRYLRPVSTAATLNFAATLAQS
ARLWKDYDPTFAADCLEKAEIAWQAALKHPDIYAEYTPGSGGPGGGPYND
DYVGDEFYWAACELYVTTGKDEYKNYLMNSPHYLEMPAKMGGEDNGLWGC
FTWGTTQGLGTITLALVENGLPATDIQKARNNIAKAADRWLENIEEQGYR
LPIKQAEDERGGYPWGSNSFILNQMIVMGYAYDFTGDSKYLDGMFDGISY
LLGRNAMDQSYVTGYGERPLQNPHDRFWTPQTSKRFPAPPPGIISGGPNS
RFEDPTINAAVKKDTPPQKCFIDHTDSWGTNQITVNWNAPFAWVTAYLDE
QY

3D structure

• PDB: 1rq5 Structural basis for the exocellulase activity of the cellobiohydrolase CbhA from Clostridium thermocellum
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D383 D386 Y555 Q795
• Catalytic site (residue number reindexed from 1): D176 D179 Y348 Q582
• Enzyme Commision number: 3.2.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	A	W473 T474 L476 S547	W266 T267 L269 S340
BS02	BGC	A	W473 G546 H737 R739 Q795	W266 G339 H524 R526 Q582
BS03	BGC	A	G553 Y555 W678 Q795	G346 Y348 W465 Q582
BS04	BGC	A	E606 W616	E393 W403
BS05	CA	A	D557 Y559 D562 E563 G617	D350 Y352 D355 E356 G404
BS06	CA	A	D421 E428 Q429 N431 Y433 D435 D438	D214 E221 Q222 N224 Y226 D228 D231

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0008810 cellulase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:1rq5, PDBe:1rq5, PDBj:1rq5
• PDBsum: 1rq5
• PubMed: 14756552
• UniProt: Q59325