Structure of PDB 1rgl Chain A

Receptor sequence

>1rglA (length=104) Species: 5062 (Aspergillus oryzae)

ACDYTCGSNCYSSSDVSTAQAAGYKLHEDGETVGSNSYPHKYNNYQGFDF
SVSSPYYEWPILSSGDVYSGGSPGADRVVFNENNQLAGVITHTGASGNNF
VECT

3D structure

• PDB: 1rgl RNase T1 mutant Glu46Gln binds the inhibitors 2'GMP and 2'AMP at the 3' subsite.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y38 H40 E58 D76 R77 T91 H92 F100
• Catalytic site (residue number reindexed from 1): Y38 H40 E58 D76 R77 T91 H92 F100
• Enzyme Commision number: 4.6.1.24: ribonuclease T1.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	2GP	A	N36 Y38 H40 E58 R77 H92 G97	N36 Y38 H40 E58 R77 H92 G97	MOAD: Kd=37uM PDBbind-CN: -logKd/Ki=4.43,Kd=37uM

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004540 RNA nuclease activity
• GO:0016829 lyase activity
• GO:0046589 ribonuclease T1 activity

Biological Process

• GO:0008150 biological_process

Cellular Component

• GO:0001411 hyphal tip
• GO:0030428 cell septum

External links

• PDB: RCSB:1rgl, PDBe:1rgl, PDBj:1rgl
• PDBsum: 1rgl
• PubMed: 1350642
• UniProt: P00651|RNT1_ASPOR Guanyl-specific ribonuclease T1 (Gene Name=rntA)